Genomic DNA isolation from dried blood using gelatin-coated magnetic particles

Sorasak Intorasoot; Jintara Techateerawat; Amornrat Intorasoot

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Genomic DNA isolation from dried blood using gelatin-coated magnetic particles

机译：使用涂有明胶的磁性颗粒从干血中分离基因组DNA

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Gelatin-coated magnetic particles were implemented for isolation of human genomic DNA from dried blood spotted onto glass microfibre filters. The DNA quality and quantity were compared to a silica-based membrane purification kit. Conditions for driedblood storage were varied for DNA stability determination. Approximately three times higher DNA yield was recovered by coated magnetic particles than the silica-based kit. In addition, the quality of extracted DNA was determined using /?-actin gene amplification by polymerase chain reaction (PCR). It was implied that genomic DNA was stable for at least 28 days in a dried condition. Gelatin-coated magnetic particles are rapid, simple and useful for DNA extraction in field applications.

机译：实施明胶包被的磁性颗粒用于从点到玻璃微纤维过滤器上的干血中分离人基因组DNA。将DNA质量和数量与基于二氧化硅的膜纯化试剂盒进行比较。改变干血保存的条件以测定DNA的稳定性。被涂覆的磁性颗粒回收的DNA产量比基于二氧化硅的试剂盒高约三倍。另外，使用β-肌动蛋白基因通过聚合酶链反应（PCR）扩增来确定提取的DNA的质量。这暗示基因组DNA在干燥条件下稳定至少28天。涂明胶的磁性颗粒快速，简单并且可用于现场应用中的DNA提取。

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《Current Science: A Fortnightly Journal of Research》 |2013年第1期|共4页
作者
Sorasak Intorasoot; Jintara Techateerawat; Amornrat Intorasoot;
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原文格式 PDF
正文语种 eng
中图分类自然科学现状及发展;
关键词
Dried blood; DNA extraction; gelatin; magnetic particles;

机译：干血;DNA提取;明胶;磁性颗粒;

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1. Genomic DNA isolation from dried blood using gelatin-coated magnetic particles [J] . Sorasak Intorasoot, Jintara Techateerawat, Amornrat Intorasoot Current Science: A Fortnightly Journal of Research . 2013,第1期

机译：使用涂有明胶的磁性颗粒从干血中分离基因组DNA
2. Application of gelatin-coated magnetic particles for isolation of genomic DNA from bacterial cells [J] . Intorasoot S, Srirung R, Intorasoot A, Analytical Biochemistry: An International Journal of Analytical and Preparative Methods . 2009,第2期

机译：明胶包覆的磁性颗粒在细菌细胞中分离基因组DNA的应用
3. Gelatin-coated magnetic nanoparticles for genomic DNA isolation from biological samples [J] . Current Science: A Fortnightly Journal of Research . 2019,第11期

机译：Gelatin涂覆的磁性纳米粒子用于生物样品的基因组DNA分离
4. Automated high-throughput purification of genomic DNA from whole blood using Promega's MagneSil~(TM) paramagnetic particles with either the Max Yield or MagneSil~(TM) ONE normalized purification methods [C] . Rex M. Bitner, Susan C. Koller, Jacqui Sankbeil Society of Photo-Optical Instrumentation Engineers Conference on Microarrays and Combinatorial Technologies for Biomedical Applications :Design, Fabrication, and Analysis . 2003

机译：使用Promega的镁〜（TM）顺磁颗粒从全血的自动化高通量纯化，MAX产量或磁头杆菌〜（TM）一种标准化纯化方法
5. Isolation and characterization of genomic DNA sequences that enhance the stability of plasmid DNA in mammalian cells. [D] . Manocha, Marcus M. S. 2006

机译：增强哺乳动物细胞中质粒DNA稳定性的基因组DNA序列的分离和表征。
6. Isolation of human genomic DNA for genetic analysis from premature neonates: a comparison between newborn dried blood spots whole blood and umbilical cord tissue [O] . Shavanthi Rajatileka, Karen Luyt, Manal El-Bokle, 2013

机译：分离人类基因组DNA进行早产儿的遗传分析：新生儿干血斑全血和脐带组织之间的比较
7. Isolation of human genomic DNA for genetic analysis from premature neonates: a comparison between newborn dried blood spots, whole blood and umbilical cord tissue [O] . 2013

机译：分离人类基因组DNA进行早产儿的遗传分析：新生儿干血斑，全血和脐带组织之间的比较

Genomic DNA isolation from dried blood using gelatin-coated magnetic particles

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