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首页> 外文期刊>Current Science: A Fortnightly Journal of Research >Detergent-induced male sterility and bud pollination in Brassica juncea (L.) Czern & Coss
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Detergent-induced male sterility and bud pollination in Brassica juncea (L.) Czern & Coss

机译：芥菜油（L.）Czern＆Coss的洗涤剂诱导的雄性不育和花粉授粉

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摘要

A large number of chemicals capable of selectively inhibiting pollen development have been identified as chemicalhybridizing agents for large-scale commercial production of hybrid seeds in various crops. These chemicals are known to cause a range of effects, for example, feminization of male florets, abnormal development of reproductive organs, inhibition of early anther development, abnormal tapetal behaviour and suppression of microspore development. The present paper reports the changes in floral biology, pollen fertility, seed-set and total yield in Brassica juncea plants sprayed with various concentrations of surf-excel- a commonly used detergent. The seeds of B. juncea (L.) Czern & Coss var. Pusa bold were sown in a randomized row design at the Botanic Garden, School of Life Sciences, Dr B. R. Ambedkar University, Agra. A group of twenty-five plants in each row were sprayed only once (a week before the floral bud initiation, i.e. 21 days after sowing) with 2, 4 and 6 percent (w/v) surf-excel (synthetic detergent powder, Hindustan Lever Ltd, Mumbai); another group of 25 plants were sprayed with distilled water to serve as control. There were three replicates for each treatment. Two rows of 25 plants each were left untreated between a row of treated plants. A small beehive was placed in the experimental plot. Pollen fertility of treated and untreated plants was tested regularly throughout the flowering period at an interval of 24 h with FCR test, 1 percent TTC (tetrazolium chloride) in 0.15 M Tris-HC1 buffer at pH 7.8, and by in vitro pollen germination test after Brewbaker and Kwack; in vivo pollen germination in the pistil was verified by the procedure after Shivanna and Rangaswami4. The inflorescence of treated and untreated plants was bagged before making these tests.
机译：已经鉴定出许多能够选择性抑制花粉发育的化学物质作为化学杂交剂，用于在各种作物中大规模商业生产杂交种子。已知这些化学物质会引起多种作用，例如，雄性小花女性化，生殖器官异常发育，抑制早期花药发育，异常绒毛行为和抑制小孢子发育。本文报道了喷施不同浓度的surf-excel（一种常用洗涤剂）的芸苔属植物的花生物学，花粉育性，结实率和总产量的变化。 B. juncea（L.）Czern＆Coss var。的种子。普萨·布鲁德（Pusa bold）在阿格拉（Agra）生命科学学院生命科学学院植物园的植物园中随机种植。每行中的一组二十五个植物仅喷一次（在花蕾萌芽前一周，即在播种后21天），然后喷洒2％，4％和6％（w / v）的surf-excel（合成洗涤剂粉，印度斯坦）孟买Lever Ltd）;另一组的25株植物用蒸馏水喷雾作为对照。每种处理重复三次。在一排经处理的植物之间，不进行处理的两排25株植物。在实验区中放置了一个小蜂箱。在整个开花期以24小时的间隔定期测试经过处理和未经处理的植物的花粉育性，方法是FCR测试，在0.15 M Tris-HCl缓冲液中，pH 7.8的1％TTC（氯化四氮唑）和接种后的体外花粉萌发测试布鲁贝克和夸克; Shivanna和Rangaswami4之后的程序验证了雌蕊体内的花粉萌发。在进行这些测试之前，将处理过的和未处理过的植物的花序装袋。

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