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首页> 外文期刊>The Analyst: The Analytical Journal of the Royal Society of Chemistry: A Monthly International Publication Dealing with All Branches of Analytical Chemistry >Real-time CRP detection from whole blood using micropost-embedded microfluidic chip incorporated with label-free biosensor
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Real-time CRP detection from whole blood using micropost-embedded microfluidic chip incorporated with label-free biosensor

机译:使用微型嵌入式微流体芯片的全血实时CRP检测,其包含无标签的生物传感器

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摘要

We demonstrate the detection of C-creative protein (CRP) from whole blood samples without sample pretreatment by using a lab-on-a-chip system consisting of a microfluidic chip and a label-free biosensor. The microfluidic chip includes an array of microposts for filtering blood cells and allows only plasma to flow through and reach the guided-mode resonance (GMR) biosensor for real-time monitoring. The developed GMR sensor can achieve a bulk sensitivity of 186 nm RIU-1, which supports a limit of detection of 3.2 ng mL(-1) for recombinant CRP spiked in human serum. The results are comparable with those obtained using enzyme-linked immunosorbent assay. In addition, we demonstrate the efficacy of filtration of blood cells using microposts and simultaneous measurement of CRP concentration using a GMR sensor by using whole blood and plasma samples.
机译:我们通过使用由微流体芯片和无标记生物传感器组成的芯片系统,证明了从整个血液样品中检测来自全血样品的C创造蛋白(CRP),没有样品预处理。 微流体芯片包括用于过滤血细胞的微型阵列,并且仅允许等离子体流过并到达导向模式共振(GMR)生物传感器以进行实时监测。 发达的GMR传感器可以实现186nm Riu-1的批量灵敏度,其支持在人血清中掺入的重组CRP的3.2ng ml(-1)的检测限。 结果与使用酶联免疫吸附测定获得的结果相当。 此外,我们证明了使用Microposts过滤血细胞的疗效,并通过使用全血和血浆样品使用GMR传感器同时测量CRP浓度。

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