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首页> 外文期刊>Bioscience, Biotechnology, and Biochemistry >PiggyBac Transposon-Mediated Gene Transfer in Cashmere Goat Fetal Fibroblast Cells
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PiggyBac Transposon-Mediated Gene Transfer in Cashmere Goat Fetal Fibroblast Cells

机译：PiggyBac转座子介导的羊绒山羊胎儿成纤维细胞中的基因转移

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摘要

PiggyBac (PB) has recently been found to be functional in various organisms. To verify and exploit its application in the cashmere goat, a PB transposon system including donor and helper vector of was developed, in which the EGFP gene in donor of vector was used as reporter. Cashmere goat fetal fibroblasts cells (GFFs) were transfected with the PB transposon system and the efficiency of gene transfer was determined. Compared with random integration, PB-medi-ated EGFP expression levels increased 7.78-fold in the GFFs, confirming that the PB transposon system constructed successfully mediated efficient foreign gene integration in the GFFs. To further investigate the characteristics of PB-mediated integration instance, PB integration site distribution in the goat genome was examined. The results showed that PB had a preference for AT rich regions of the goat genome. Thus this study confirms the function of PB transposon in GFFs and provides a potential genetic tool for producing transgenic goats.
机译：最近发现，PiggyBac（PB）在多种生物中具有功能。为了验证和开发其在羊绒山羊中的应用，开发了一个PB转座子系统，包括供体和辅助载体，其中载体供体中的EGFP基因被用作报告基因。用PB转座子系统转染羊绒山羊胎儿成纤维细胞（GFF），并确定基因转移的效率。与随机整合相比，PB介导的EGFP在GFF中的表达水平增加了7.78倍，这证实了PB转座子系统在GFF中成功地介导了有效的外源基因整合。为了进一步研究PB介导的整合实例的特征，检查了山羊基因组中PB整合位点的分布。结果表明，PB偏爱山羊基因组的AT富集区域。因此，这项研究证实了PB转座子在GFF中的功能，并为生产转基因山羊提供了潜在的遗传工具。

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