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首页> 外文期刊>Biosensors & Bioelectronics: The International Journal for the Professional Involved with Research, Technology and Applications of Biosensers and Related Devices >Rapid detection of influenza A virus infection utilizing an immunomagnetic bead-based microfluidic system
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Rapid detection of influenza A virus infection utilizing an immunomagnetic bead-based microfluidic system

机译：利用基于免疫磁珠的微流体系统快速检测甲型流感病毒感染

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This study reports a new immunomagnetic bead-based microfluidic system for the rapid detection of influenza A virus infection by performing a simple two-step diagnostic process that includes a magnetic bead-based fluorescent immunoassay (FIA) and an end-point optical analysis. With the incorporation of monoclonal antibody (mAb)-conjugated immunomagnetic beads, target influenza A viral particles such as A/H_1N_1 and A/H_3N_2 can be specifically recognized and are bound onto the surface of the immunomagnetic beads from the specimen sample. This is followed by labeling the fluorescent signal onto the virus-bound magnetic complexes by specific developing mAb with R-phycoerythrin (PE). Finally, the optical intensity of the magnetic complexes can be analyzed immediately by the optical detection module. Significantly, the limit of detection (LOD) of this immunomagnetic bead-based microfluidic system for the detection of influenza A virus in a specimen sample is approximately 5×10~(-4) hemagglutin units (HAU), which is 1024 times better than compared to conventional bench-top systems using flow cytometry. More importantly, the entire diagnostic protocol, from the purification of target viral particles to optical detection of the magnetic complexes, can be automatically completed within 15min in this immunomagnetic bead-based microfluidic system, which is only 8.5% of the time required when compared to a manual protocol. As a whole, this microfluidic system may provide a powerful platform for the rapid diagnosis of influenza A virus infection and may be extended for diagnosis of other types of infectious diseases with a high specificity and sensitivity.
机译：这项研究报告了一种新的基于免疫磁珠的微流控系统，该系统可通过执行简单的两步诊断过程（包括基于磁珠的荧光免疫测定（FIA）和终点光学分析）来快速检测甲型流感病毒感染。通过掺入结合有单克隆抗体（mAb）的免疫磁珠，可以特异性识别目标A型流感病毒颗粒，例如A / H_1N_1和A / H_3N_2，并将其结合到样本样品的免疫磁珠表面。随后，用带有R-藻红蛋白（PE）的特异性发育mAb将荧光信号标记到病毒结合的磁性复合物上。最后，可以通过光学检测模块立即分析磁性配合物的光学强度。重要的是，这种基于免疫磁珠的微流系统检测标本中甲型流感病毒的检出限（LOD）约为5×10〜（-4）血凝素单位（HAU），比其高出1024倍与使用流式细胞仪的传统台式系统相比。更重要的是，在这种基于免疫磁珠的微流控系统中，从目标病毒颗粒的纯化到磁性复合物的光学检测的整个诊断方案可以在15分钟内自动完成，而与手动协议。总体而言，该微流体系统可以为快速诊断甲型流感病毒提供强大的平台，并且可以扩展为以高特异性和敏感性来诊断其他类型的传染病。

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