...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Vaccine >Novel ssRNA phage VLP platform for displaying foreign epitopes by genetic fusion
【24h】

Novel ssRNA phage VLP platform for displaying foreign epitopes by genetic fusion

机译：新型SSRNA噬菌体VLP平台用于通过遗传融合显示外部表位

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Virus-like particles (VLPs) can be used as efficient carriers of various antigens and therefore serve as attractive tools in vaccine development. Although VLPs of different viruses can be used, VLPs of ssRNA phages have convincing advantages due to their unique properties, including efficient protein production in bacterial and yeast expression systems, low production cost and easy and fast purification. Currently, the range of ssRNA phage VLPs is limited. In particular, this is true for VLPs that tolerate insertions at the N- and C-termini of the coat protein. It is therefore necessary to find new alternatives within the known ssRNA phage VLP range. From previous studies, we found approximately 80 new VLPs forming ssRNA phage coat proteins. In the current study, we attached a model peptide to the N- and C-termini of coat proteins. As a model peptide, we used a triple repeat of 23 N-terminal residues of the ectodomain of the influenza M2 protein, used previously in the development of the flu vaccine. Examining 43 novel phage coat proteins for the ability to form chimeric VLPs, we found ten new promising candidates for further vaccine design, five of which were tolerant to insertions at both the N- and C-termini. Furthermore, we demonstrate that most of the chimeric VLPs have good antigenic properties as judged from their reactivity with anti-M2 antibodies. (C) 2020 Elsevier Ltd. All rights reserved.
机译：病毒样颗粒（VLP）可被用作各种抗原的有效载流子，并且因此作为疫苗开发有吸引力的工具。尽管可以使用不同的病毒的VLP，的ssRNA噬菌体的VLP的已令人信服优点由于其独特的性质，包括高效率生产蛋白质在细菌和酵母表达系统，生产成本低，容易和快速纯化。目前，单链RNA噬菌体病毒样颗粒的范围是有限的。特别地，这是为使得在外壳蛋白的N-和C-末端容忍插入的VLP真。因此，有必要找出已知的单链RNA噬菌体VLP范围内新的替代品。从以往的研究中，我们发现约80新病毒样颗粒形成单链RNA噬菌体外壳蛋白。在目前的研究中，我们附着模型肽外壳蛋白的N-和C-末端。作为一名模特肽，我们使用的流感病毒M2蛋白的胞外域的23 N端残基的三重重复，在流感疫苗的发展以前使用的。检查43个新颖噬菌体外壳蛋白为以形成嵌合VLP的能力，我们发现进行进一步的疫苗设计十个新希望的候选，其中五个是耐受在N-和C-末端插入。此外，我们证明了大部分的嵌合VLP具有良好的抗原性从他们的反应与抗M2抗体判断。 （c）2020 elestvier有限公司保留所有权利。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号