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首页> 外文期刊>Dalton transactions: An international journal of inorganic chemistry >Thermostable enzyme-immobilized magnetic responsive Ni-based metal-organic framework nanorods as recyclable biocatalysts for efficient biosynthesis of S-adenosylmethionine
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Thermostable enzyme-immobilized magnetic responsive Ni-based metal-organic framework nanorods as recyclable biocatalysts for efficient biosynthesis of S-adenosylmethionine

机译：热稳定的酶固定化磁性响应性Ni基金属 - 有机框架纳米棒作为可回收生物催化剂，用于高效的S-腺苷甲硫氨酸生物合成

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摘要

A novel magnetic responsive Ni-based metal-organic framework material was developed to efficiently separate and immobilize thermal enzymes with high catalytic performance. Ni-based metal-organic framework nanorods (Fe3O4/Ni-BTC) with high magnetic responsiveness are prepared conveniently by a one-pot hydrothermal process. With the characterization, it was confirmed that Ni-based metal-organic framework nanocomposites were synthesized as nanorods mounted with magnetic Fe3O nanoparticles on the surface. Although Fe3O4/Ni-BTC showed a preference for histidine-tagged enhanced green fluorescent protein (His-eGFP), we found that a variety of forces played roles in enzyme immobilization, including affinity between Ni2+ and histidine tags, electrostatic attraction, hydrogen bonding and hydrophobic forces. After understanding the mechanism of the as-prepared nanocomposites, a new immobilization strategy for thermostable S-adenosylmethionine synthetase (SAMS) was further evaluated. As a result, SAMS from cell lysate achieved about 95% activity recovery in the biosynthesis of S-adenosylmethionine (SAM) under high temperature conditions (70 degrees C) with a simple mixing step. At the same time, the immobilized enzyme was more stable against temperature variation (by nearly 8-fold in an 80 degrees C water bath after 2 h) and extreme pH (by nearly 1.3-fold at pH 3) and exhibited excellent reusability after immobilization. This work indicates that magnetic responsive Ni-based nanorods are highly promising for thermostable enzyme immobilization with high efficiency and stability.
机译：开发了一种新型磁性响应Ni的金属 - 有机骨架材料，以有效地分离和固定具有高催化性能的热酶。基于Ni的金属 - 有机框架纳米棒（Fe3O4 / Ni-BTC）具有高磁反应性的高磁性反应性通过单罐水热法制备。凭借表征，证实基于Ni的金属 - 有机骨架纳米复合材料被合成为安装在表面上的磁性Fe3O纳米颗粒的纳米棒。虽然Fe3O4 / Ni-BTC显示出对组氨酸标记的增强的绿色荧光蛋白（HIS-EGFP）的偏好，但我们发现各种力量在酶固定中发挥作用，包括Ni2 +和组氨酸标签之间的亲和力，静电吸引力，氢键和氢键合并疏水力。在理解AS制备的纳米复合材料的机制之后，进一步评价了用于热稳定的S-腺苷甲基乙硫氨酸合成酶（SAMS）的新的固定策略。结果，来自细胞裂解物的SAM在高温条件下（70℃）在高温条件下（70℃）在高温条件下实现了约95％的活性恢复。同时，固定化酶的温度变化更稳定（在2小时后80℃水浴中近8倍），极端pH（在pH 3下近1.3倍），并在固定后发出优异的可重用性。该作品表明，磁性响应性Ni的纳米棒具有高效率和稳定性的热稳定酶固定性的高度令人令人无希望。

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    He Jie; Sun Shanshan; Zhou Zhao; Yuan Qipeng; Liu Yanhui; Liang Hao;

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    Beijing Univ Chem Technol State Key Lab Chem Resource Engn Beijing 100029 Peoples R China;

    Beijing Univ Chem Technol State Key Lab Chem Resource Engn Beijing 100029 Peoples R China;

    Beijing Univ Chem Technol State Key Lab Chem Resource Engn Beijing 100029 Peoples R China;

    Beijing Univ Chem Technol State Key Lab Chem Resource Engn Beijing 100029 Peoples R China;

    Beijing Univ Chem Technol State Key Lab Chem Resource Engn Beijing 100029 Peoples R China;

    Beijing Univ Chem Technol State Key Lab Chem Resource Engn Beijing 100029 Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;无机化学;
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