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首页> 外文期刊>Dalton transactions: An international journal of inorganic chemistry >Rapid and highly sensitive detection of extracellular and intracellular H2S by an azide-functionalized Al(III)-based metal-organic framework
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Rapid and highly sensitive detection of extracellular and intracellular H2S by an azide-functionalized Al(III)-based metal-organic framework

机译：通过叠氮化物功能化的Al（III）的金属 - 有机框架快速和高敏感的细胞外和细胞内H2S检测

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摘要

A new, azide-functionalized Al(III)-based metal-organic framework (MOF) denoted as CAU-10-N-3 (1, CAU = Christian-Albrechts-University) and consisting of the 5-azido-isophthalic acid (H(2)IPA-N-3) ligand was employed as a reaction-based fluorescent turn-on probe for the detection of H2S. The activated compound (1') showed fast, selective and highly sensitive sensing properties for extracellular H2S in HEPES buffer (10 mM, pH = 7.4). The material retained its high selectivity even in the presence of possibly competing biological species. The limit of detection of 1' for H2S is 2.65 mu M, which is lower than the earlier reports on MOFs for H2S sensing. The material displayed a short response time (420 s) and a significant increase (20-fold and 26-fold after 1 and 7 min of addition of Na2S, respectively) in the fluorescence intensity towards H2S. Macrophage cells loaded with probe 1' exhibited blue fluorescence with a response time of 15 min after Na2S addition, indicating the suitability of the probe for intracellular H2S detection. Moreover, CAU-10-N-3 featured excellent detection performance (quick response and 32-fold increment in fluorescence intensity after 7 min of Na2S addition) in water. Hence, it can be utilized to regulate the H2S level in aqueous samples collected from the environment.
机译：基于Cau-10-N-3（1，Cau = Christian-Albrechts-University的新的叠氮化物官能化的Al（III）的金属有机框架（MOF），并由5-厌氮 - 间苯二甲酸组成（ H（2）IPA-N-3）配体作为基于反应的荧光匝开启探针，用于检测H2S。活化的化合物（1'）在HEPES缓冲液（10mM，pH = 7.4）中为细胞外H2S显示出快速，选择性和高敏感的感测性能。即使在可能竞争的生物物种存在下，该材料也能保留其高选择性。 H2S的检测限为2.65 mu m，低于H2S感测的早期报告。该材料显示出短响应时间（420 s），并且在荧光强度朝H2s中荧光强度分别在1和7分钟后的20倍和26倍。用探针1'负载巨噬细胞的巨噬细胞，在Na 2 S的添加后，在15分钟的响应时间上表现出蓝色荧光，表明探针对细胞内H2S检测的适用性。此外，CAU-10-N-3在水中具有出色的检测性能（在Na 2 S加入7分钟后，荧光强度的快速响应和32倍的增量）。因此，它可用于调节从环境收集的水性样品中的H2S水平。

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