...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Development >A collection of genetic mouse lines and related tools for inducible and reversible intersectional mis-expression
【24h】

A collection of genetic mouse lines and related tools for inducible and reversible intersectional mis-expression

机译：遗传鼠标线的集合和诱导诱导和可逆交叉表达的相关工具

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Thanks to many advances in genetic manipulation, mouse models have become very powerful in their ability to interrogate biological processes. In order to precisely target expression of a gene of interest to particular cell types, intersectional genetic approaches using two promoter/enhancers unique to a cell type are ideal. Within these methodologies, variants that add temporal control of gene expression are the most powerful. We describe the development, validation and application of an intersectional approach that involves three transgenes, requiring the intersection of two promoter/enhancers to target gene expression to precise cell types. Furthermore, the approach uses available lines expressing tTA/rTA to control the timing of gene expression based on whether doxycydine is absent or present, respectively. We also show that the approach can be extended to other animal models, using chicken embryos. We generated three mouse lines targeted at the Tigre (Igs7) locus with TRE-loxP-tdTomato-loxP upstream of three genes (p21, DTA and Ctgf), and combined them with Cre and tTA/rtTA lines that target expression to the cerebellum and limbs. Our tools will facilitate unraveling biological questions in multiple fields and organisms.
机译：由于在遗传操作的许多进展，小鼠模型已经在自己的能力来询问的生物过程变得非常强大。为了感兴趣的特定细胞类型，使用两个启动子交叉遗传方法的基因的精确目标表达/增强特有的细胞类型是理想的。这些方法中，也添加基因表达的时空控制的变体是最强大的。我们描述了包括三个转基因，需要两个启动子/增强子的交点到靶基因的表达，以精确的细胞类型的交叉方法的开发，确认和应用。此外，该方法使用表达tTA的/ RTA控制基因表达的基于定时doxycydine是否是不存在或存在，分别可用线路。我们还表明，该方法可以用鸡胚可以扩展到其他动物模型。我们产生了针对与三个基因（p21基因，DTA和CTGF）TRE-loxP的tdTomato-loxP序列上游蒂格雷（Igs7）位点3个小鼠品系，并用酶Cre和tTA的/的rtTA线组合他们，目标表达小脑和四肢。我们的工具将有助于解开在多个领域和生物的生物学问题。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号