UNIT 3.15 Isolation of Lipid Droplets from Cells by Density Gradient Centrifugation

Dawn L. Brasaemle; Nathan E. Wolins

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UNIT 3.15 Isolation of Lipid Droplets from Cells by Density Gradient Centrifugation

机译：UNIT 3.15通过密度梯度离心从细胞中分离脂滴

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Lipid droplets are organelles found in most mammalian cells, as well as in various plant tissues and yeast. They are composed of a core of neutral lipids surrounded by a membrane monolayer of phospholipids and cholesterol in which specific proteins are embedded. This unit provides protocols for isolating lipid droplets from mammalian cells by discontinuous density gradient centrifugation.

机译：脂滴是在大多数哺乳动物细胞以及各种植物组织和酵母菌中发现的细胞器。它们由被脂质和胆固醇的膜单层包围的中性脂质的核心组成，其中嵌入了特定的蛋白质。该单元提供了通过不连续密度梯度离心从哺乳动物细胞中分离脂滴的方案。

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《Current Protocols in Cell Biology》 |2016年第73期|共13页
作者
Dawn L. Brasaemle; Nathan E. Wolins;
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正文语种 eng
中图分类细胞生物学;
关键词
cell cultures; mammalian; preparation of lipid droplets by centrifugation; density-gradient centrifugation; isolation of lipid droplets;

机译：细胞培养;哺乳动物;离心制备脂质滴;密度梯度离心;脂质滴分离;

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1. UNIT 3.15 Isolation of Lipid Droplets from Cells by Density Gradient Centrifugation [J] . Dawn L. Brasaemle, Nathan E. Wolins Current Protocols in Cell Biology . 2016,第Suppla73期

机译：UNIT 3.15通过密度梯度离心从细胞中分离脂滴
2. Microfluidic Adaptation of Density-Gradient Centrifugation for Isolation of Particles and Cells [J] . Yuxi Sun, Palaniappan Sethu Bioengineering . 2017,第3期

机译：密度梯度离心微流控技术用于分离颗粒和细胞
3. Density Gradient Centrifugation for the Isolation of Cells of Multiple Lineages [J] . Yamamoto Yumiko, Itoh Shousaku, Yamauchi Yukako, Journal of cellular biochemistry. . 2015,第12期

机译：密度梯度离心分离多个谱系的细胞
4. Detection of Circulating Tumor Cells in Blood Using an Optimized Density Gradient Centrifugation [C] . Ralf Gertler, Michael Dahm, Katrin Fuehrer, International Meeting on Molecular Staging on Cancer . 2003

机译：使用优化密度梯度离心检测血液中循环肿瘤细胞
5. An ER-dependent lipid droplet formation model—A subcellular localization study of multiple proteins involved in lipid droplet biogenesis, and, Lipid droplet association of RDH10 and its functional study [D] . Jiang, Weiya 2008

机译：依赖ER的脂质滴形成模型—涉及脂质滴生物发生的多种蛋白质的亚细胞定位研究以及RDH10的脂质滴缔合及其功能研究
6. Isolation of Lipid Droplets from Cells by Density Gradient Centrifugation [O] . Dawn L. Brasaemle, Nathan E. Wolins -1

机译：通过密度梯度离心从细胞中分离脂质滴
7. Isolation of T cell-replacing factor (TRF) responsive B lymphoblastoid cells by percoll density gradient centrifugation [O] . Akihito Ishizaka, Yukio Sakiyama, Motohiko Okano, 1984

机译：通过Percoll密度梯度离心分离T细胞替代因子（TRF）响应性B淋巴母细胞的分离
8. Changes in Canine Neutrophil Function(s) Following Cellular Isolation by Percoll Gradient Centrifugation or Isotonic Lysis [R] . Gruber, D. F., D'Alesandro, M. M. 1988

机译：percoll梯度离心或等渗裂解细胞分离后犬中性粒细胞功能的变化

UNIT 3.15 Isolation of Lipid Droplets from Cells by Density Gradient Centrifugation

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