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首页> 外文期刊>Current Protocols in Cell Biology >UNIT 25.3 Three-Dimensional Patterning of the ECM Microenvironment Using Magnetic Nanoparticle Self Assembly
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UNIT 25.3 Three-Dimensional Patterning of the ECM Microenvironment Using Magnetic Nanoparticle Self Assembly

机译：UNIT 25.3使用磁性纳米粒子自组装对ECM微环境进行三维图案化

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This protocol describes a way to introduce topography to three-dimensional (3D) biomaterials. The self-assembling behavior of magnetic particles can be exploited to form nanoscale to microscale fibers, such that one can dissect the contribution of topography on cell behavior, which is independent of other physical properties of the biomaterial (e.g., stiffness). The magnetic particles are chemically cross-linked with several extracellular matrix (ECM) proteins and then using magnetic force-mediated assembly, one can program aligned nanofibers in a 3D hydrogel. This process allows the creation of diverse topographic patterns in 3D, including isotropic, anisotropic (fibril), or interfaced architectures, without changing the bulk stiffness of the scaffold material. This anisotropic architecture guides the dendritic protrusions of cells, which can be compared to cells grown in an isotropic architecture lacking spatial guidance cues. Several cell types, such as fibroblasts and neurons, have been cultured in this engineered 3D matrix. This technology provides an easy way to construct nano-bio interfaces for various biomedical engineering applications as well as dissect the role of topography in various cell behaviors.
机译：该协议描述了一种将地形引入三维（3D）生物材料的方法。可以利用磁性粒子的自组装行为来形成纳米级到微米级的纤维，使得人们可以解剖形貌对细胞行为的贡献，这与生物材料的其他物理特性（例如刚度）无关。磁性粒子与几种细胞外基质（ECM）蛋白化学交联，然后使用磁力介导的组装，可以在3D水凝胶中编程对齐的纳米纤维。此过程可在不更改支架材料的整体刚度的情况下，以3D形式创建各种拓扑图案，包括各向同性，各向异性（原纤维）或界面体系结构。这种各向异性的体系结构指导细胞的树突状突起，可以与缺乏空间引导线索的各向同性体系结构中生长的细胞进行比较。在这种工程化的3D矩阵中已经培养了几种细胞类型，例如成纤维细胞和神经元。这项技术为构建用于各种生物医学工程应用的纳米生物界面以及剖析形貌在各种细胞行为中的作用提供了一种简便的方法。

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