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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Systematic Characterization of the Metabolism of Acetoin and Its Derivative Ligustrazine in Bacillus subtilis under Micro-Oxygen Conditions
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Systematic Characterization of the Metabolism of Acetoin and Its Derivative Ligustrazine in Bacillus subtilis under Micro-Oxygen Conditions

机译：微氧条件下枯草芽孢杆菌及其衍生川芎嗪的系统性质表征

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摘要

Bacillus subtilis is an important microorganism for brewing of Chinese Baijiu, which contributes to the formation of flavor chemicals including acetoin and its derivative ligustrazine. The first stage of Baijiu brewing process is under micro oxygen conditions; however, there are few studies about B. subtilis metabolism under these conditions. Effects of various factors on acetoin and ligustrazine metabolism were investigated under these conditions, including key genes and fermentation conditions. Mutation of bdhA (encoding acetoin reductase) or overexpression of glcU (encoding glucose uptake protein) increased acetoin concentration. Addition of Vigna angularis powder to the culture medium also promoted acetoin production. Optimal culture conditions for ligustrazine synthesis were pH 6.0 and 42 degrees C. Ammonium phosphate was shown to promote ligustrazine synthesis in situ. This is the first report of acetoin and ligustrazine metabolism in B. subtilis under micro-oxygen conditions, which will ultimately promote the application of B. subtilis for maintaining Baijiu quality.
机译：枯草芽孢杆菌是酿造中国白九的重要微生物，这有助于形成含有丙酮蛋白及其衍生川芎嗪的风味化学品。 Baijiu Brewing Process的第一阶段是微氧条件;然而，在这些条件下，关于B.枯草芽孢杆菌代谢的研究很少。在这些条件下研究了各种因素对丙酮素和川芎嗪代谢的影响，包括关键基因和发酵条件。 BDHA（编码乙酰蛋白还原酶）或Glcu（编码葡萄糖摄取蛋白）的过表达增加丙酮素浓度。向培养基中添加豇豆Angularis粉末也促进了乙酰蛋白的产生。 Ligustrazine合成的最佳培养条件是pH6.0和42℃。显示磷酸铵以促进川芎嗪合成原位。这是微氧条件下B.枯草芽孢杆菌的第一份丙酮蛋白和川芎嗪代谢的报告，最终将促进B.枯草芽孢杆菌的应用来维持Baijiu质量。

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  • 来源
  • 作者

    Xu Youqiang; Jiang Yuefeng; Li Xiuting; Sun Baoguo; Teng Chao; Yang Ran; Xiong Ke; Fan Guangsen; Wang Wenhua;

  • 作者单位

    Beijing Technol &

    Business Univ Beijing Adv Innovat Ctr Food Nutr &

    Human Hlth Beijing 100048 Peoples R China;

    Beijing Technol &

    Business Univ Beijing Adv Innovat Ctr Food Nutr &

    Human Hlth Beijing 100048 Peoples R China;

    Beijing Technol &

    Business Univ Beijing Adv Innovat Ctr Food Nutr &

    Human Hlth Beijing 100048 Peoples R China;

    Beijing Technol &

    Business Univ Beijing Adv Innovat Ctr Food Nutr &

    Human Hlth Beijing 100048 Peoples R China;

    Beijing Technol &

    Business Univ Beijing Engn &

    Technol Res Ctr Food Addit Beijing 100048 Peoples R China;

    Beijing Technol &

    Business Univ Beijing Engn &

    Technol Res Ctr Food Addit Beijing 100048 Peoples R China;

    Beijing Technol &

    Business Univ Beijing Adv Innovat Ctr Food Nutr &

    Human Hlth Beijing 100048 Peoples R China;

    Beijing Technol &

    Business Univ Beijing Adv Innovat Ctr Food Nutr &

    Human Hlth Beijing 100048 Peoples R China;

    Beijing Technol &

    Business Univ Beijing Adv Innovat Ctr Food Nutr &

    Human Hlth Beijing 100048 Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 营养卫生、食品卫生;农业科学;
  • 关键词

    Bacillus subtilis; acetoin; ligustrazine; micro-oxygen conditions; Chinese Baijiu;

    机译：枯草芽孢杆菌;丙酮蛋白;川芎嗪;微氧气条件;中国白津;

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