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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Development of Yam Dioscorin-Loaded Nanoparticles for Paracellular Transport Across Human Intestinal Caco-2 Cell Monolayers
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Development of Yam Dioscorin-Loaded Nanoparticles for Paracellular Transport Across Human Intestinal Caco-2 Cell Monolayers

机译：亚马逊素纳米粒子的开发用于人类肠道Caco-2细胞单层的肺膜状肺癌

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摘要

Dioscorins, the major storage proteins of yam tubers, exert immunomodulatory activities. To improve oral bioavailability of dioscorins in the intestine, recombinant dioscorin (rDioscorin) was coated with N,N,N-trimethyl chitosan (TMC) and tripolyphosphate (TPP), resulting in the formation of TMC-rDio-TPP nanoparticles (NPs). The loading capacity and entrapment efficiency of rDioscorin in the NPs were 26 +/- 0.7% and 61 +/- 1.4%, respectively. The NPs demonstrated a substantial release profile in the pH environment of the jejunum. The rDioscorin released from the NPs stimulated proliferation and phagocytosis of the macrophage RAW264.7 and activated the gene expression of IL-1 beta and IL-6. Incubation of the NPs in the Caco-2 cell monolayer led to a 5.2-fold increase of P-app compared with rDioscorin alone, suggesting that rDioscorin, with the assistance of TMC, can be promptly transported across the intestinal epithelia. These results demonstrate that the TMC-rDio-TPP NPs can be utilized for elucidating the immunopharmacological effects of dioscorins through oral delivery.
机译：Dioscorins，山药块的主要储存蛋白，施加免疫调节活动。为了提高肠道二磷酸素的口服生物利用度，重组二磷素（Rdioscorin）（Rdioscorin）用N，N，N-三甲基壳聚糖（TMC）和三聚磷酸酯（TPP）涂覆，导致TMC-RdiO-TPP纳米颗粒（NPS）的形成。 NPS中Rdioscorin的负载能力和夹带效率分别为26 +/- 0.7％和61 +/- 1.4％。 NPS在JEJUNUM的pH环境中显示出大量释放曲线。从NPS刺激的RDIOSCORIN释放出巨噬细胞RAW264.7的刺激增殖和吞噬作用，并激活IL-1β和IL-6的基因表达。与单独的Rdioscorin相比，在CaCO-2细胞单层中的NPS在CaCO-2细胞单层中的温育导致p-app的5.2倍，表明Rdioscorin随着TMC的辅助，可以在肠上皮内迅速运输。这些结果表明，TMC-RdiO-TPP NPS可用于阐明Dioscorins通过口服递送的免疫药业作用。

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