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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Hydrolysis of Lactose and Transglycosylation of Selected Sugar Alcohols by LacA beta-Galactosidase from Lactobacillus plantarum WCFS1
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Hydrolysis of Lactose and Transglycosylation of Selected Sugar Alcohols by LacA beta-Galactosidase from Lactobacillus plantarum WCFS1

机译：Lacaβ-半乳糖苷来自Lactobacillus Plantarum WCFS1的Laca Beta-半乳糖苷酶的乳糖和异膜化水解

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摘要

The production, biochemical characterization, and carbohydrate specificity of LacA beta-galactosidase (locus lp_3469) belonging to the glycoside hydrolase family 42 from the probiotic organism Lactobacillus plantarum WCFS1 are addressed. The beta-D-galactosidase activity was maximal in the pH range of 4.0-7.0 and at 30-37 degrees C. High hydrolysis capacity toward the beta(1 -> 4) linkages between galactose and glucose (lactose) or fructose (lactulose) was found. High efficiency toward galactosyl derivative formation was observed when lactose and glycerol, xylitol, or erythritol were used. Galactosyl derivatives of xylitol were characterized for the first time as 3-O-beta-D-galactopyranosyl-xylitol and 1-O-beta-D-galactopyranosyl-xylitol, displaying high preference of LacA beta-galactosidase for the transfer of galactosyl residues from lactose to the C1 or C3 hydroxyl group of xylitol. These results indicate the feasibility of using LacA beta-galactosidase for the synthesis of different galactosyl-polyols, which could be promising candidates for beneficial and appealing functional and technological applications such as novel prebiotics or hypocaloric sweeteners.
机译：解决了属于益生菌生物乳酸盐菌WCFS1的糖苷水解酶系列42的Lacaβ-半乳糖苷酶（基因糖LP_3469）的生产，生化表征和碳水化合物特异性。 β-D-半乳糖苷酶活性在4.0-7.0的pH范围内最大，在30-37℃下，在半乳糖和葡萄糖（乳糖）或果糖（乳糖）之间的β（1-> 4）键的高水解容量被找到。使用乳糖和甘油，木糖醇或赤藓糖醇时观察到半乳糖基衍生物形成的高效率。三霉素的半乳糖基衍生物首次表征为3-O-β-D-半乳糖醇基 - 木糖醇和1-β-D-半乳糖醇 - 木糖醇，显示LACAβ-半乳糖苷酶的高偏好，用于转移半乳糖基残留物乳糖到木糖醇的C1或C3羟基。这些结果表明使用Lacaβ-半乳糖苷酶用于合成不同半乳糖基 - 多元醇的可行性，这可能是有利和吸引力的功能和技术应用的有希望的候选者，例如新的益生元或低丙基甜味剂。

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