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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Harnessing Adaptive Evolution to Achieve Superior Mannitol Production by Lactococcus lactis Using Its Native Metabolism
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Harnessing Adaptive Evolution to Achieve Superior Mannitol Production by Lactococcus lactis Using Its Native Metabolism

机译：利用自适应进化以实现乳酸乳酸乳酸的优越甘露醇生产，利用其原生新陈代谢

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摘要

Mannitol can be obtained as a by-product of certain heterolactic lactic acid bacteria, when grown on substrates containing fructose. Lactococcus lactis, a homolactic lactic acid bacterium, normally does not form mannitol but can be persuaded into doing so by expressing certain foreign enzyme activities. In this study, we find that L. lactis has an inherent capacity to form mannitol from glucose. By adaptively evolving L. lactis or derivatives blocked in NAD(+) regenerating pathways, we manage to accelerate growth on mannitol. When cells of the adapted strains are resuspended in buffer containing glucose, 4-58% of the glucose metabolized is converted into mannitol, in contrast to nonadapted strains. The highest conversion was obtained for a strain lacking all major NAD(+) regenerating pathways. Mannitol had an inhibitory effect on the conversion, which we speculated was due to the mannitol uptake system. After its inactivation, 60% of the glucose was converted into mannitol by cells suspended in glucose buffer. Using a two-stage setup, where biomass first was accumulated by aerated culturing, followed by a nonaerated phase (static conditions), it was possible to obtain 6.1 g/L mannitol, where 60% of the glucose had been converted into mannitol, which is the highest yield reported for L. lactis.
机译：甘露醇可以如某些heterolactic乳酸菌的副产物，在含有果糖衬底上生长时获得。乳酸乳球菌，一个同型乳酸细菌，通常不形成甘露醇但可以说服到通过表达某些外国酶活性这样做。在这项研究中，我们发现，乳酸乳球菌具有固有能力，以葡萄糖的形式甘露醇。通过自适应地不断变化的乳酸乳球菌或阻塞在NAD（+）衍生物再生途径，我们设法加快甘露醇生长。当重新悬浮在缓冲液中含有葡萄糖的适应的菌株的细胞，代谢葡萄糖的4-58％被转化为甘露糖醇，在对比nonadapted菌株。为缺乏所有主要的NAD（+）再生途径的菌株，获得最高的转换。甘露醇对转换，我们推测是由于甘露醇吸收系统的抑制作用。其失活后，将葡萄糖的60％是由悬浮在缓冲液中的葡萄糖的细胞转化为甘露糖醇。使用两阶段的设置，其中生物质第一被充气培养积累，随后进行非曝气阶段（静态条件），这是能够得到6.1克/ L甘露糖醇，其中所述葡萄糖的60％已被转化为甘露糖醇，其是报道的乳酸乳球菌的最高产量。

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