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首页> 外文期刊>Journal of Applied Polymer Science >Fabrication and evaluation of poly(lactic acid), chitosan, and tricalcium phosphate biocomposites for guided bone regeneration
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Fabrication and evaluation of poly(lactic acid), chitosan, and tricalcium phosphate biocomposites for guided bone regeneration

机译：聚（乳酸），壳聚糖和磷酸三钙生物复合材料的制备和评价，用于引导骨再生

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ABSTRACT > This study presents and evaluates an approach for fabricating poly(lactic acid) (PLA)/chitosan (CS)/tricalcium phosphate (TCP) electrospun scaffolds for guided bone regeneration, a dental procedure that uses membranes to direct and delineate regions of osteogenesis. Biomaterials were pre‐processed using cryomilling, a solid‐state grinding technique that facilitates the generation of powdered biocomposites conducive to electrospinning. X‐ray diffraction (XRD) confirmed the generation of cryomilled blends consisting of PLA, CS, and TCP. Results from the differential scanning calorimetry showed an upward shift in glass transition temperature and an increase in crystallinity with the inclusion of TCP reinforcing the observations from XRD. Murine macrophages were used to confirm the biocompatibility of the cryomilled powders and was evaluated using CellTiter‐Blue (CTB) cell viability assay and brightfield microscopy. Scanning electron microscopy was used to examine the morphology of the fibers produced via electrospinning, while Raman spectroscopy confirmed material homogeneity. In vitro studies with MG‐63 cells validated the capacity of composite scaffolds to encourage proliferation, while Coherent anti‐Stokes Raman scattering and fluorescence microscopies provided visual evidence of cell proliferation. CTB assay revealed that cells maintain viability and metabolic activity at 3 and 7 days after seeding, demonstrating the potential of the biocomposite membranes. ? 2018 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2018 , 135 , 46692. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract XMLNS =“http://www.wiley.com/namespaces/wiley”type =“main”> <title type =“main”>抽象</ title> >本研究显示并评估了一种制造方法聚（乳酸）（PLA）/壳聚糖（CS）/磷酸三钙（TCP）电纺支架，用于引导骨再生，一种使用膜直接和描绘成骨区域的牙科手术。使用冷冻机预处理生物材料，一种固态研磨技术，其有助于产生有利于静电纺丝的粉末化生物复合材料。 X射线衍射（XRD）证实了由PLA，CS和TCP组成的低温混合物。来自差示扫描量热法的结果显示玻璃化转变温度向上偏移，并在XRD中包含TCP的结晶度的增加。使用鼠巨噬细胞来证实冷冻粉的生物相容性，并使用细胞 - 蓝（CTB）细胞活力测定和BrightField显微镜评估。扫描电子显微镜用于检查通过静电纺丝产生的纤维的形态，而拉曼光谱证实材料均匀性。 <I>体外</ i>替换MG-63细胞的研究证实复合支架的能力，以鼓励增殖，而相干反斯托克斯拉曼散射和荧光显微术提供细胞增殖的视觉证据。 CTB检测显示，细胞在接种后3和7天保持活力和代谢活性，证明生物复合膜的电位。还是2018 Wiley期刊，Inc.J.Phill。聚合物。 SCI。 2018 </ b>， 135 </ i>，46692。</ p> </ abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-33581/'>《Journal of Applied Polymer Science》</a> <b style="margin: 0 2px;">|</b><span>2018年第40期</span><b style="margin: 0 2px;">|</b><span>共10页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Ramesh Srikanthan&option=202" target="_blank" rel="nofollow">Ramesh Srikanthan;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Lungaro Lisa&option=202" target="_blank" rel="nofollow">Lungaro Lisa;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Tsikritsis Dimitrios&option=202" target="_blank" rel="nofollow">Tsikritsis Dimitrios;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Weflen Eric&option=202" target="_blank" rel="nofollow">Weflen Eric;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Rivero Iris V.&option=202" target="_blank" rel="nofollow">Rivero Iris V.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Elfick Alistair P. D.&option=202" target="_blank" rel="nofollow">Elfick Alistair P. D.;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Department of Industrial and Manufacturing Systems EngineeringIowa State UniversityAmes Iowa 50011;</p> <p>Institute for Bioengineering University of Edinburgh Mayfield RoadEdinburgh EH9 3DW UK;</p> <p>Institute for Bioengineering University of Edinburgh Mayfield RoadEdinburgh EH9 3DW UK;</p> <p>Department of Industrial and Manufacturing Systems EngineeringIowa State UniversityAmes Iowa 50011;</p> <p>Department of Industrial and Manufacturing Systems EngineeringIowa State UniversityAmes Iowa 50011;</p> <p>Institute for Bioengineering University of Edinburgh Mayfield RoadEdinburgh EH9 3DW UK;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/1821.html" title="高分子化合物工业（高聚物工业）">高分子化合物工业（高聚物工业）;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=biomaterials&option=203" rel="nofollow">biomaterials;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=biomedical applications&option=203" rel="nofollow">biomedical applications;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=blends&option=203" rel="nofollow">blends;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=composites&option=203" rel="nofollow">composites;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=fibers&option=203" rel="nofollow">fibers;</a> </p> <div class="translation"> 机译：生物材料;生物医学应用;混合;复合材料;纤维; 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