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首页> 外文期刊>Journal of Cell Science >Passive diffusion through nuclear pore complexes regulates levels of the yeast SAGA and SLIK coactivator complexes
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Passive diffusion through nuclear pore complexes regulates levels of the yeast SAGA and SLIK coactivator complexes

机译：通过核孔隙复合物的被动扩散调节酵母佐贺和Slik共粘膜复合物的水平

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摘要

Nuclear pore complexes (NPCs) control gene expression by regulating the bi-directional exchange of proteins and RNAs between nuclear and cytoplasmic compartments, including access of transcriptional regulators to the nucleoplasm. Here, we show that the yeast (Saccharomyces cerevisiae) nucleoporin Nup170, in addition to binding and silencing subtelomeric genes, supports transcription of genes regulated by the SAGA transcriptional activator complex. Specifically, we show that a lower amount of SAGA complex is bound to target genes in the absence of Nup170. Consistent with this observation, levels of the SAGA complex are decreased in cells lacking Nup170, while those of the SAGA-related SLIK complexes are increased. This change in the ratio of SAGA to SLIK complexes is due to increased nuclear activity of Pep4, a protease responsible for production of the SLIK complex. Further analyses of various nucleoporin mutants revealed that the increased nuclear entry of Pep4 observed in the nup170 Delta mutant likely occurs as the consequence of an increase in the sieving limits of the NPC diffusion channel. On the basis of these results, we propose that changes in passive diffusion rates represent a mechanism for regulating SAGA- and SLIK complex-mediated transcriptional events.
机译：通过调节核和细胞质隔室之间的双向交换蛋白质和RNA的双向交换，包括转录调节剂到核状的核孔隙络合物（NPCS）控制基因表达。在这里，我们表明酵母（酿酒酵母酿酒酵母）核致素NUP170除了结合和沉默的子细胞键基因之外，还支持由SAGA转录活化剂复合物调节的基因的转录。具体地，我们表明，在没有NUP170的情况下，较低量的佐菌络合物将与靶基因结合。与该观察结果一致，在缺乏NUP170的细胞中，SAGA复合物的水平降低，而佐贺相关的SLIK复合物的细胞增加。这种变化的佐贺与Slik复合物的比例是由于Pep4的核活动增加，该蛋白酶负责生产Slik络合物。进一步分析各种核偶蛋白突变体揭示了在NUP170 Delta突变体中观察到的Pep4的核纳入增加可能是由于NPC扩散通道的筛分界限的增加而发生的。在这些结果的基础上，我们提出了被动扩散速率的变化代表了调节佐贺和Slik复合介导的转录事件的机制。

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