• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Molecular Biology >Effects of DNA Superhelical Stress on the Stability of H2B-Ubiquitylated Nucleosomes
【24h】

Effects of DNA Superhelical Stress on the Stability of H2B-Ubiquitylated Nucleosomes

机译：DNA超标性应力对H2B-UbiquityLated核肉稳定性的影响

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

On the nucleosome level, histone posttranslational modifications function mainly as the regulatory signals; in addition, some posttranslational modifications can enhance nucleosome stochastic folding, which is restricted in "canonic" nucleosomes. Recently, it has been shown in vitro that symmetric or asymmetric nucleosome ubiquitylation at H2BK34 (and H2BK120, to a lesser extent) can destabilize one of the nucleosomal H2A-H2B dimers and promote nucleosome conversion to a hexasome particle [Krajewski et al. (2018). Nucleic Acids Res., 46, 7631-7642]. Such lability of H2Bub nucleosomes raises a question of whether they could accommodate transient changes in DNA torsional tensions, which are generated by virtually any process that manipulates DNA strands. Using positively or negatively supercoiled DNA minicircles and homogeneously-modified H2Bub histones, we have found that DNA topology could strongly and selectively affect nucleosome stability depending on its ubiquitylation state (here the term "nucleosome stability" means the nucleosome property to maintain its structural integrity and dynamics characteristic to "canonic" nucleosomes). The results point to a role for H2B ubiquitylation in amplifying or mitigating the effects of a DNA torque on the nucleosome stability and dynamics. (C) 2018 Elsevier Ltd. All rights reserved.
机译：在核细胞体水平上，组蛋白后期改性主要是调节信号;此外，一些后期改变的修饰可以增强核小体随机折叠，其受到“官方”核肉中的限制。最近，已经在体外示出了H2BK34（和H2BK120的对称或不对称核心菌液，可以使核体H 2-H 2B二聚体中的一种变得破坏核致组H 2-H 2B二聚体，并促进核心转化率至己大体粒子[Krajewski等人。 （2018）。核酸RES，46,761-7642]。这种H2Bub核体的这种易持态度提出了它们是否能适应DNA扭转张力的瞬时变化，这几乎是一种操纵DNA链的方法。使用阳性或负面超底DNA小碱基和均匀改性的H2BUB组蛋白，我们发现DNA拓扑结构根据其泛醌态（这里术语“核心稳定性”是指保持其结构完整性的核心属性和核心稳定性动态特征为“官僚”核心）。结果指出了H2B泛力扩增或减轻DNA扭矩对核小体稳定性和动力学的影响的作用。 （c）2018年elestvier有限公司保留所有权利。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号