• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Acta biomaterialia >Development of hyaluronic acid-based scaffolds for brain tissue engineering.
【24h】

Development of hyaluronic acid-based scaffolds for brain tissue engineering.

机译：基于透明质酸的脑组织工程支架的开发。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Three-dimensional biodegradable porous scaffolds play vital roles in tissue engineering. In this study, a hyaluronic acid-collagen (HA-Coll) sponge with an open porous structure and mechanical behavior comparable to brain tissue was developed. HA-Coll scaffolds with different mixing ratios were prepared by a freeze-drying technique and crosslinked with water-soluble carbodiimide to improve mechanical stability. The pore structure of the samples was evaluated by light and scanning electron microscopy, and the mechanical behavior was analyzed by mechanical compression and tension testing. The degree of crosslinking was determined by the water absorption and trinitrobenzene sulfonic assay, and the HA content was determined by a carbazole assay. The results showed that HA-Coll scaffolds containing an open porous structure with a homogeneous pore size distribution could be fabricated. Certain features of the mechanical properties of HA-Coll scaffolds prepared with a Coll:HA mixing ratio of 1:2, and pure HA sponges, were comparable with brain tissue. Neural stem cells (NSCs) were expanded in number in monolayer culture and then seeded onto the three-dimensional scaffolds in order to investigate the effects of the different types of scaffolds on neurogenic induction of the cells. This study contributes to the understanding of the effects of HA content and crosslink treatment on pore characteristics, and mechanical behavior essential for the design of HA-Coll scaffolds suitable for NSC growth and differentiation for brain tissue engineering.
机译：三维可生物降解的多孔支架在组织工程中起着至关重要的作用。在这项研究中，开发了一种透明质酸胶原蛋白（HA-Coll）海绵，该海绵具有与大脑组织相当的开孔结构和机械性能。通过冷冻干燥技术制备具有不同混合比的HA-Coll支架，并与水溶性碳二亚胺交联以提高机械稳定性。通过光镜和扫描电子显微镜评价样品的孔结构，并通过机械压缩和拉伸试验分析机械性能。通过吸水和三硝基苯磺酸测定法确定交联度，并且通过咔唑测定法确定HA含量。结果表明，可以制备具有开放的多孔结构且孔径分布均匀的HA-Coll支架。以Coll：HA混合比为1：2制备的HA-Coll支架和纯HA海绵的力学性能的某些特征与脑组织相当。神经干细胞（NSCs）在单层培养中扩增数量，然后接种到三维支架上，以研究不同类型支架对细胞神经源性诱导的影响。这项研究有助于了解HA含量和交联处理对孔特征的影响，以及对于设计适合于NSC生长和脑组织工程分化的HA-Coll支架所必需的机械行为。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号