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首页> 外文期刊>Acta biomaterialia >Morphological and functional studies of rat hepatocytes on a hydrophobic or hydrophilic polydimethylsiloxane surface.
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Morphological and functional studies of rat hepatocytes on a hydrophobic or hydrophilic polydimethylsiloxane surface.

机译：疏水或亲水聚二甲基硅氧烷表面上大鼠肝细胞的形态学和功能研究。

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摘要

This study describes the morphological and functional behavior of rat hepatocytes on a polydimethylsiloxane (PDMS)-coated surface. Hepatocytes were cultured on hydrophobic or hydrophilic PDMS-coated surfaces in serum-free and serum-containing media. In the serum-free medium, almost all hepatocytes adhered onto the surface irrespective of the wettability, with a cell adhesion ratio of >90% at 24h. In the serum-containing medium, although they strongly adhered onto the hydrophilic surface (cell adhesion ratio >85%), the ratio on the hydrophobic surface was <15%. Furthermore, hepatocytes in the serum-free medium gradually formed spheroids irrespective of the surface characteristics; however, on the hydrophilic surface in the serum-containing medium, they maintained a monolayer configuration for up to 10 days, and their numbers gradually decreased over time. Expression levels of the functional activities (albumin secretion and ammonia removal) and the cell-cell adhesion molecules (cadherin and connexin-32) were higher in the hepatocytes that formed spheroids compared to those which assumed a monolayer configuration, and these levels were maintained for at least 10 days. These results suggest that the wettability of PDMS and the composition of the culture medium together control the cell adhesion, morphology and expression of functional genes in hepatocytes.
机译：这项研究描述了在聚二甲基硅氧烷（PDMS）涂层表面上大鼠肝细胞的形态和功能行为。在无血清和含血清的培养基中，在疏水或亲水的PDMS涂层表面上培养肝细胞。在无血清培养基中，几乎所有肝细胞都粘附在表面上，而与润湿性无关，在24h时细胞粘附率> 90％。在含血清的培养基中，尽管它们牢固地粘附在亲水表面上（细胞粘附率> 85％），但在疏水表面上的比率却<15％。此外，无血清培养基中的肝细胞逐渐形成球体，而与表面特性无关。然而，在含血清培养基中的亲水性表面上，它们保持单层结构长达10天，并且其数量随着时间的推移逐渐减少。与呈单层构型的肝细胞相比，形成球体的肝细胞的功能活性（白蛋白分泌和氨去除）和细胞间粘附分子（钙黏着蛋白和连接蛋白-32）的表达水平更高，并且这些水平保持了一定水平。至少10天。这些结果表明PDMS的润湿性和培养基的组成共同控制肝细胞中细胞的粘附，形态和功能基因的表达。

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