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首页> 外文期刊>Acta biomaterialia >Cellular response to nanoscale elastin-like polypeptide polyelectrolyte multilayers.
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Cellular response to nanoscale elastin-like polypeptide polyelectrolyte multilayers.

机译：细胞对纳米级弹性蛋白样多肽聚电解质多层的反应。

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Ionic elastin-like polypeptide (ELP) conjugates are a new class of biocompatible, self-assembling biomaterials. ELPs composed of the repeat unit (GVGVP)(n) are derived from the primary sequence of mammalian elastin and produced in Escherichia coli. These biopolymers exhibit an inverse transition temperature that renders them extremely useful for applications in cell-sheet engineering. Cationic and anionic conjugates were synthesized by the chemical coupling of ELP to polyethyleneimine (PEI) and polyacrylic acid (PAA). The self-assembly of ELP-PEI and ELP-PAA using the layer-by-layer deposition of alternately charged polyelectrolytes is a simple, versatile technique to generate bioactive and biomimetic surfaces with the ability to modulate cell-substratum interactions. Our studies are focused on cellular response to self-assembled multilayers of ionic (GVGVP)(40) incorporated within the polymeric sequence H(2)N-MVSACRGPG-(GVGVP)(40)-WP-COOH. Angle-dependent XPS studies indicated a difference in the chemical composition at the surface ( approximately 10A below the surface) and subsurface regions. These studies provided additional insight into the growth of the nanoscale multilayer assembly as well as the chemical environment that the cells can sense. Overall, cellular response was enhanced on glass substrata coated with ELP conjugates compared with uncoated surfaces. We report significant differences in cell proliferation, focal adhesions and cytoskeletal organization as a function of the number of bilayers in each assembly. These multilayer assemblies have the potential to be successfully utilized in the rational design of coatings on biomaterials to elicit a desired cellular response.
机译：离子弹性蛋白样多肽（ELP）偶联物是一类新的生物相容性，自组装生物材料。由重复单元（GVGVP）（n）组成的ELP源自哺乳动物弹性蛋白的一级序列，并在大肠杆菌中产生。这些生物聚合物表现出逆转变温度，这使其在细胞片工程中的应用极为有用。通过ELP与聚乙烯亚胺（PEI）和聚丙烯酸（PAA）的化学偶联合成了阳离子和阴离子共轭物。使用交替充电的聚电解质的逐层沉积进行ELP-PEI和ELP-PAA的自组装是一种简单，通用的技术，可以生成具有生物活性和仿生表面并具有调节细胞-基质相互作用的能力。我们的研究集中于细胞对结合到聚合物序列H（2）N-MVSACRGPG-（GVGVP）（40）-WP-COOH中的离子（GVGVP）（40）的自组装多层的反应。与角度相关的XPS研究表明，表面（表面以下约10A）和地下区域的化学成分有所不同。这些研究为纳米级多层组件的生长以及细胞可以感知的化学环境提供了更多的见识。总体而言，与未涂覆的表面相比，在涂覆有ELP共轭物的玻璃基质上，细胞反应得到了增强。我们报告细胞增殖，粘着斑和细胞骨架组织的显着差异作为每个程序中双层数的函数。这些多层组件具有成功用于生物材料涂层合理设计以引发所需细胞反应的潜力。

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