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首页> 外文期刊>Acta biomaterialia >Regulation of the protein-loading capacity of hydroxyapatite by mercaptosuccinic acid modification.
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Regulation of the protein-loading capacity of hydroxyapatite by mercaptosuccinic acid modification.

机译：通过巯基琥珀酸修饰调节羟基磷灰石的蛋白质负载能力。

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摘要

Loading and releasing protein in a controllable way is extremely important for the protein vehicles used in bone tissue engineering. To obtain a suitable carrier material for basic proteins, such as BMP or bFGF, hydroxyapatite particles containing mercaptosuccinic acid (mercaptosuccinic acid (Mer), (Mer-HAp)) were synthesized. Physicochemical evaluation of Mer-HAp suggested that Mer was contained in HAp particles: it either simply adsorbed onto HAp crystals or was trapped among the HAp crystals. A protein adsorption study using basic and acidic model proteins indicated that the synthesized Mer-HAp had selective loading properties of the basic protein. The loaded protein was gradually released from Mer-HAp in phosphate buffered saline. The protein release rate was different in each Mer-HAp synthesized with a different concentration of Mer. In addition, the protein release from Mer-HAp showed a similar profile with the Ca dissolution in different pH solutions, indicating that the Mer-HAp dissolution was concerned with the protein release from Mer-HAp. Thus, Mer-HAp is a useful candidate for the basic protein carrier because it has properties which enable the loading and releasing of protein in a controllable way.
机译：对于骨骼组织工程中使用的蛋白质载体，以可控制的方式加载和释放蛋白质极为重要。为了获得适合碱性蛋白（例如BMP或bFGF）的载体材料，合成了含有巯基琥珀酸（巯基琥珀酸（Mer），（Mer-HAp））的羟基磷灰石颗粒。 Mer-HAp的理化评价表明，Mer包含在HAp颗粒中：它要么简单地吸附到HAp晶体上，要么被捕获在HAp晶体中。使用碱性和酸性模型蛋白进行的蛋白质吸附研究表明，合成的Mer-HAp具有碱性蛋白的选择性负载特性。负载的蛋白质在磷酸缓冲盐溶液中从Mer-HAp逐渐释放。在使用不同浓度的Mer合成的每种Mer-HAp中，蛋白质的释放速率均不同。此外，从Mer-HAp释放的蛋白质显示出与在不同pH溶液中的Ca溶解相似的曲线，这表明Mer-HAp的溶解与从Mer-HAp释放的蛋白质有关。因此，Mer-HAp是碱性蛋白载体的有用候选物，因为它具有能够以可控方式加载和释放蛋白的特性。

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