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首页> 外文期刊>Acta biomaterialia >Perichondrium directed cartilage formation in silk fibroin and chitosan blend scaffolds for tracheal transplantation.
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Perichondrium directed cartilage formation in silk fibroin and chitosan blend scaffolds for tracheal transplantation.

机译：绢丝蛋白和壳聚糖混合支架中的软骨膜定向软骨形成，用于气管移植。

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摘要

The purpose of this study was to investigate the potential of silk fibroin and chitosan blend (SFCS) biological scaffolds for the purpose of cartilage tissue engineering with applications in tracheal tissue reconstruction. The capability of these scaffolds as cell carrier systems for chondrocytes was determined in vitro and cartilage generation in vivo on engineered chondrocyte-scaffold constructs with and without a perichondrium wrapping was tested in an in vivo nude mouse model. SFCS scaffolds supported chondrocyte adhesion, proliferation, and differentiation, determined as features of the cells based on the spherical cell morphology, increased accumulation of glycosaminoglycans, and increased collagen type II deposition with time within the scaffold framework. Perichondrium wrapping significantly (P<0.001) improved chondrogenesis within the cell-scaffold constructs in vivo. In vivo implantation for 6weeks did not generate cartilage structures resembling native trachea, although cartilage-like structures were present. The mechanical properties of the regenerated tissue increased due to the deposition of chondrogenic matrix within the SFCS scaffold structural framework of the trachea. The support of chondrogenesis by the SFCS tubular scaffold construct resulted in a mechanically sound structure and thus is a step towards an engineered trachea that could potentially support the growth of an epithelial lining resulting in a tracheal transplant with properties resembling those of the fully functional native trachea.
机译：这项研究的目的是研究丝素蛋白和壳聚糖共混物（SFCS）生物支架在软骨组织工程中的潜力及其在气管组织重建中的应用。在体外确定这些支架作为软骨细胞的细胞载体系统的能力，并在体内裸鼠模型中测试在有和没有软骨膜包裹的工程软骨细胞支架构建体上的体内软骨生成。 SFCS支架支持软骨细胞的粘附，增殖和分化，这是根据球形细胞形态确定的细胞特征，糖胺聚糖的积累增加以及II型胶原在支架框架内随时间的沉积增加。软骨膜包裹显着（P <0.001）改善了体内细胞支架构建体内的软骨形成。尽管存在类似软骨的结构，但六周的体内植入并未产生类似于天然气管的软骨结构。由于软骨基质在气管的SFCS支架结构框架内的沉积，增强了再生组织的机械性能。 SFCS管状支架构造支持软骨形成，形成了机械稳固的结构，因此是朝工程气管迈出的一步，可以潜在地支持上皮内膜的生长，从而导致气管移植，其性质类似于完全功能的天然气管。 。

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