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首页> 外文期刊>Acta biomaterialia >Protein adsorption on thin films of carbon and carbon nitride monitored with in situ ellipsometry.
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Protein adsorption on thin films of carbon and carbon nitride monitored with in situ ellipsometry.

机译：用原位椭偏法监测蛋白质在碳和氮化碳薄膜上的吸附。

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Thin films of amorphous carbon and amorphous, graphitic and fullerene-like carbon nitride were deposited by reactive magnetron sputtering and optically characterized with spectroscopic ellipsometry. Complementary studies using scanning electron microscopy and atomic force microscopy were performed. The films were exposed to human serum albumin (HSA) and the adsorption was monitored in situ using dynamic ellipsometry. From the ellipsometric data the adsorbed amount of proteins was quantified in terms of surface mass density using de Feijter's model. The results indicate larger adsorption of proteins onto the amorphous films compared to the films with a more textured structure. Complementary studies with 125I-labeled HSA showed an apparent protein adsorption up to six times larger compared to the ellipsometry measurement. In addition, the four types of films were incubated in blood plasma followed by exposure to anti-fibrinogen, anti-HMWK or anti-C3c, revealing the materials' response to complement and contact activation. The amorphous and graphitic carbon nitride exhibit rather high immune activity compared to a titanium reference, whereas the amorphous carbon and the fullerene-like CNx show less immune complement deposition. Compared to the reference, all films exhibit indications of a stronger ability to initiate the intrinsic pathway of coagulation. Finally, the surfaces' bone-bonding ability was investigated by examination of their ability to form calcium phosphate crystals in a simulated body fluid, with a-CNx depositing most calcium phosphate after 21 days of incubation.
机译：通过反应磁控溅射沉积非晶碳以及非晶，石墨和富勒烯状氮化碳的薄膜，并通过椭圆偏振光谱法对其进行光学表征。使用扫描电子显微镜和原子力显微镜进行了补充研究。将膜暴露于人血清白蛋白（HSA），并使用动态椭圆偏振法原位监测吸附。根据椭偏数据，使用de Feijter模型根据表面质量密度对蛋白质的吸附量进行了定量。结果表明与具有更多纹理结构的膜相比，蛋白质在非晶膜上的吸附更大。用125 I标记的HSA进行的补充研究表明，与椭圆光度法测量相比，表观蛋白质的吸附量最多高出六倍。此外，将四种类型的薄膜在血浆中孵育，然后暴露于抗纤维蛋白原，抗HMWK或抗C3c，揭示了材料对补体和接触活化的反应。与钛参考相比，无定形和石墨氮化碳显示出相当高的免疫活性，而无定形碳和类似富勒烯的CNx显示出较少的免疫补体沉积。与参考文献相比，所有薄膜均显示出更强的引发内在凝血途径的能力。最后，通过检查表面在模拟体液中形成磷酸钙晶体的能力来研究其表面的骨结合能力，在培养21天后，α-CNx沉积了大部分磷酸钙。

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