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首页> 外文期刊>Acta biomaterialia >Sulfated hyaluronan/collagen I matrices enhance the osteogenic differentiation of human mesenchymal stromal cells in vitro even in the absence of dexamethasone.
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Sulfated hyaluronan/collagen I matrices enhance the osteogenic differentiation of human mesenchymal stromal cells in vitro even in the absence of dexamethasone.

机译：即使在没有地塞米松的情况下，硫酸化透明质酸/胶原I基质也能在体外增强人间充质基质细胞的成骨分化。

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摘要

Glycosaminoglycans (GAG) are multifunctional components of the extracellular matrix (ECM) involved in different steps of the regulation of cellular differentiation. In this study artificial extracellular matrices (aECM) consisting of collagen (Col) I and different GAG derivatives were used as a substrate for human mesenchymal stromal cells (hMSC) to study osteogenic differentiation in vitro. hMSC were cultured on aECM containing col and hyaluronan sulfates (HyaS) with increasing degrees of sulfation (DS(S)) and were compared with aECM containing col and the natural GAG hyaluronan or chondroitin 4-sulfate. hMSC were analyzed for osteogenic differentiation markers such as calcium phosphate deposition, tissue non-specific alkaline phosphatase (TNAP) and expression of runt-related transcription factor 2 (runx2), osteocalcin (ocn) and bone sialoprotein II (bspII). Compared with aECM containing Col and natural GAG all Col/HyaS-containing aECM induced an increase in calcium phosphate deposition, TNAP activity and tnap expression. These effects were also seen in the absence of dexamethasone (an established osteogenic supplement). The expression of runx2 and ocn was not altered and the expression of bspII was diminished on the col/HyaS-containing aECM. The impact of the Col/HyaS-containing aECM on hMSC differentiation was independent of the DS(S) of the HyaS derivatives, indicating the importance of the primary (C-6) hydroxyl group of N-acetylglucosamine. These results suggest that Col/HyaS-containing aECM are able to stimulate hMSC to undergo osteogenic differentiation even in the absence of dexamethasone, which makes these matrices an interesting tool for hMSC-based tissue engineering applications and biomaterial functionalizations to enhance bone formation.
机译：糖胺聚糖（GAG）是细胞外基质（ECM）的多功能成分，参与细胞分化调节的不同步骤。在这项研究中，由胶原（Col）I和不同GAG衍生物组成的人工细胞外基质（aECM）被用作人间充质基质细胞（hMSC）的底物，以研究体外成骨细胞的分化。将hMSC在含有col和透明质酸硫酸盐（DSa）的aECM上培养，并将其与含有col和天然GAG透明质酸或4-硫酸软骨素的aECM进行比较。分析了hMSC的成骨分化标记，例如磷酸钙沉积，组织非特异性碱性磷酸酶（TNAP）和矮子相关转录因子2（runx2），骨钙素（ocn）和骨唾液蛋白II（bspII）的表达。与含有Col和天然GAG的aECM相比，所有含有Col / HyaS的aECM都导致磷酸钙沉积，TNAP活性和tnap表达增加。在没有地塞米松（已确定的成骨补充剂）的情况下，也可以看到这些效果。在含有col / HyaS的aECM上，runx2和ocn的表达没有改变，bspII的表达也减少了。含Col / HyaS的aECM对hMSC分化的影响独立于HyaS衍生物的DS（S），表明N-乙酰氨基葡糖伯（C-6）羟基的重要性。这些结果表明，即使在没有地塞米松的情况下，含有Col / HyaS的aECM仍能刺激hMSC经历成骨分化，这使这些基质成为基于hMSC的组织工程应用和生物材料功能化以增强骨骼形成的有趣工具。

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