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首页> 外文期刊>Acta biomaterialia >Artificial extracellular matrices composed of collagen i and sulfated hyaluronan with adsorbed transforming growth factor β1 promote collagen synthesis of human mesenchymal stromal cells
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Artificial extracellular matrices composed of collagen i and sulfated hyaluronan with adsorbed transforming growth factor β1 promote collagen synthesis of human mesenchymal stromal cells

机译：胶原i和硫酸透明质酸及吸附的转化生长因子β1组成的人工细胞外基质促进人间质基质细胞的胶原合成。

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摘要

Sulfated glycosaminoglycans (GAG) are multifunctional components of the extracellular matrix and are involved in the regulation of adhesion, proliferation and differentiation of cells. The effects of GAG are mediated in general by their interactions with cations and water, and in particular by their binding to growth factors. The aim of this study was to generate artificial extracellular matrices (aECM) containing collagen I and hyaluronan sulfate (HyaS), which are capable of adsorbing and releasing transforming growth factor β1 (TGF-β1), and to promote collagen synthesis of cultured human mesenchymal stromal cells (hMSC). For the preparation of aECM, monosulfated Hya (HyaS1) or trisulfated Hya (HyaS3) were used; the natural chondroitin-4-sulfate was used as a control. As applied for the in vitro experiments, the resulting matrices were composed of 93-98% collagen I and 2-7% GAG derivative. Adsorption of TGF-β1 to the aECM and release from the aECM was dependent on the degree of sulfation of hyaluronan. Collagen synthesis of hMSC was promoted only by aECM with adsorbed TGF-β1; the bare aECM had a slightly inhibitory effect on collagen synthesis. The promoting effect did not correlate either to the amount of adsorbed TGF-β1 nor to the release of TGF-β1, indicating that the correct presentation of TGF-β1 to the cells might be critical. The results indicate that sulfated hyaluronan-containing aECM have the potential to control both the adsorption and release of TGF-β1, and thereby promote collagen synthesis of hMSC. Thus, these aECM might be a useful tool for different tissue-engineering applications to enhance bone formation when used for biomaterial coating.
机译：硫酸糖胺聚糖（GAG）是细胞外基质的多功能成分，参与细胞黏附，增殖和分化的调控。一般而言，GAG的作用是通过它们与阳离子和水的相互作用，尤其是它们与生长因子的结合来介导的。这项研究的目的是生成包含胶原I和透明质酸硫酸盐（HyaS）的人工细胞外基质（aECM），它们能够吸附和释放转化生长因子β1（TGF-β1），并促进培养的人间质胶原合成基质细胞（hMSC）。为了制备aECM，使用了单硫酸化Hya（HyaS1）或三硫酸化Hya（HyaS3）;使用天然的4-硫酸软骨素作为对照。如用于体外实验，所得基质由93-98％的胶原蛋白I和2-7％的GAG衍生物组成。 TGF-β1对aECM的吸附和从aECM的释放取决于透明质酸的硫酸化程度。 hMSC的胶原蛋白合成仅通过吸附有TGF-β1的aECM来促进。裸露的aECM对胶原蛋白合成有轻微的抑制作用。促进作用与TGF-β1的吸附量或TGF-β1的释放均无关，这表明TGF-β1向细胞的正确表达可能至关重要。结果表明，硫酸化的含透明质酸的aECM具有控制TGF-β1的吸附和释放的潜力，从而促进hMSC的胶原蛋白合成。因此，当用于生物材料涂层时，这些aECM可能是用于不同组织工程应用以增强骨骼形成的有用工具。

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