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首页> 外文期刊>Acta biomaterialia >Responsive hydrogels produced via organic sol-gel chemistry for cell culture applications
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Responsive hydrogels produced via organic sol-gel chemistry for cell culture applications

机译：通过有机溶胶凝胶化学生产的用于细胞培养的响应水凝胶

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In this study, we report the synthesis of novel environmentally responsive polyurea hydrogel networks prepared via organic sol-gel chemistry and demonstrate that the networks can stabilize pH while releasing glucose both in simple aqueous media and in mammalian cell culture settings. Hydrogel formulations have been developed based on the combination of an aliphatic triisocyanate with pH-insensitive amine functional polyether and pH-sensitive poly(ethyleneimine) segments in a minimally toxic solvent suitable for the sol-gel reaction. The polyether component of the polyurea network is sufficiently hydrophilic to give rise to some level of swelling independent of environmental pH, while the poly(ethyleneimine) component contains tertiary amine groups providing pH sensitivity to the network in the form of enhanced swelling and release under acidic conditions. The reaction of these materials to form a network is rapid and requires no catalyst. The resultant material exhibits the desired pH-responsive swelling behavior and demonstrates its ability to simultaneously neutralize lactic acid and release glucose in both cell-free culture media and mammalian cell culture, with no detectable evidence of cytotoxicity or changes in cell behavior, in the case of either SA-13 human hybridomas or mouse embryonic stem cells. Furthermore, pH is observed to have a clear effect on the rate at which glucose is released from the hydrogel network. Such characteristics promise to maintain a favorable cell culture environment in the absence of human intervention.
机译：在这项研究中，我们报告了通过有机溶胶-凝胶化学制备的新型环境响应性聚脲水凝胶网络的合成，并证明了该网络可以稳定pH值，同时在简单的水介质和哺乳动物细胞培养环境中释放葡萄糖。基于脂肪族三异氰酸酯与对pH不敏感的胺官能聚醚和对pH敏感的聚亚乙基亚胺链段在适合溶胶-凝胶反应的最低毒性溶剂中的结合，已经开发出了水凝胶制剂。聚脲网络的聚醚组分具有足够的亲水性，可引起一定程度的溶胀，而与环境pH无关，而聚（乙烯亚胺）组分则含有叔胺基团，以增强的溶胀和在酸性条件下释放的形式向网络提供pH敏感性。条件。这些材料形成网络的反应迅速，不需要催化剂。所得材料显示出所需的pH响应溶胀行为，并显示出在无细胞培养基和哺乳动物细胞培养物中同时中和乳酸和释放葡萄糖的能力，在这种情况下，没有可检测到的细胞毒性或细胞行为改变的证据。 SA-13人杂交瘤或小鼠胚胎干细胞的表达。此外，观察到pH对水凝胶网络中葡萄糖的释放速率具有明显的影响。这些特征有望在没有人为干预的情况下维持良好的细胞培养环境。

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