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首页> 外文期刊>Acta biomaterialia >Modulation of osteogenic activity of BMP-2 by cellulose and chitosan derivatives
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Modulation of osteogenic activity of BMP-2 by cellulose and chitosan derivatives

机译：纤维素和壳聚糖衍生物对BMP-2成骨活性的调节

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Polysaccharides with structure and potential bioactivity similar to heparin were synthesized based on cellulose which was regioselectively sulfated, carboxylated or carboxymethylated, and chitosan that was sulfated only. Osteogenic activity of these derivatives was studied in cooperation with BMP-2 using C2C12 myoblast cells as a model system measuring alkaline phosphatase (ALP) activity and the expression of the genes Osterix, Noggin and Runx-2. It was found that highly sulfated chitosan showed the strongest osteogenic activity of all polysaccharides, but only at lower concentrations, while higher concentrations were inhibitory. By contrast, cellulose with a low or intermediate degree of sulfation showed increasing ALP activity and expression of Osterix and Noggin with rising concentrations. Lower sulfated cellulose with a high degree of carboxylation was less osteogenic, but had a positive effect on cell viability, while carboxymethylated cellulose had almost no osteogenic activity. The results indicate that regioselectively sulfated as well as carboxylated cellulose and chitosan possess an osteogenic activity, which makes them interesting candidates for application in tissue engineering of bone.
机译：基于区域选择性硫酸化，羧化或羧甲基化的纤维素和仅被硫酸化的壳聚糖，合成了具有类似于肝素的结构和潜在生物活性的多糖。使用C2C12成肌细胞作为测量碱性磷酸酶（ALP）活性以及Osterix，Noggin和Runx-2基因表达的模型系统，与BMP-2合作研究了这些衍生物的成骨活性。发现高度硫酸化的壳聚糖显示所有多糖中最强的成骨活性，但仅在较低浓度下，而较高浓度则具有抑制作用。相比之下，具有低或中度硫酸化程度的纤维素显示ALP活性增加，并且Osterix和Noggin的表达随浓度增加而增加。具有较高羧化度的低级硫酸化纤维素具有较低的成骨性，但对细胞活力具有积极作用，而羧甲基化纤维素几乎没有成骨活性。结果表明，区域选择性硫酸化以及羧化纤维素和壳聚糖具有成骨活性，这使其成为在骨组织工程中应用的有趣候选物。

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