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首页> 外文期刊>Acta biomaterialia >Cellular activity of bioactive nanograined/ultrafine-grained materials.
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Cellular activity of bioactive nanograined/ultrafine-grained materials.

机译：生物活性纳米颗粒/超细颗粒材料的细胞活性。

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Our recent electron microscopy study on biomimetic nanostructured coatings on nanograined/ultrafine-grained (NG/UFG) substrates [Mater Sci Eng C 2009;29:2417-27] indicated that electrocrystallized nanohydroxyapatite (nHA) on phase-reversion-induced NG/UFG substrates exhibited a vein-type interconnected and fibrillar structure that closely mimicked the hierarchical structure of bone. The fibrillar structure on NG/UFG substrate is expected to be more favorable for cellular response than a planar surface. In contrast, hydroxyapatite (HA) coating on coarse-grained (CG) substrate more closely resembled a film rather than a fibrillar structure. Inspired by the differences in the structure of HA coating, we describe here the cell-substrate interactions of pre-osteoblasts (MC 3T3-E1) on bioactive NG/UFG and CG austenitic stainless steel substrates. NG/UFG austenitic stainless steel was obtained by a novel controlled phase-reversion annealing of cold-deformed austenite. This example provides an illustration of how a combination of cellular and molecular biology, materials science and engineering can advance our understanding of cell-substrate interactions. Interestingly, the cellular response of nanohydroxyapatite (nHA)-coated NG/UFG substrate demonstrated superior cytocompatibility, improved initial cell attachment, higher viability and proliferation, and well-spread morphology in relation to HA-coated CG substrate and their respective uncoated (bare) counterparts as implied by fluorescence and electron microscopy and MTT assay. Similar conclusions were derived from an immunofluorescence study that involved examination of the expression levels of vinculin focal adhesion contacts associated with dense actin stress fibers and fibronectin, protein analysis through protein bands in SDS-PAGE, and quantitative total protein assay. The enhancement of cellular response followed the sequence: nHA-coated NG/UFG>nHA-coated CG>NG/UFG>CG substrates. The outcomes of the study are expected to counter the challenges associated with the engineering of nanostructured surfaces with specific physical and surface properties for medical devices with significantly improved cellular response.
机译：我们最近对纳米颗粒/超细颗粒（NG / UFG）基质上的仿生纳米结构涂层的电子显微镜研究[Mater Sci Eng C 2009; 29：2417-27]表明，在相变诱导NG / UFG上电结晶纳米羟基磷灰石（nHA）基底显示出静脉型的相互连接的原纤维结构，紧密地模仿了骨骼的层次结构。预计NG / UFG基板上的原纤维结构比平面更有利于细胞反应。相反，粗粒（CG）基材上的羟基磷灰石（HA）涂层更类似于薄膜，而不是原纤维结构。受HA涂层结构差异的启发，我们在此描述成骨细胞（MC 3T3-E1）在生物活性NG / UFG和CG奥氏体不锈钢基底上的细胞-基底相互作用。 NG / UFG奥氏体不锈钢是通过对冷变形奥氏体进行新型的控制相变退火而获得的。这个例子说明了细胞和分子生物学，材料科学和工程学的结合如何可以增进我们对细胞-底物相互作用的理解。有趣的是，纳米羟基磷灰石（nHA）涂覆的NG / UFG底物的细胞应答显示出优异的细胞相容性，改善的初始细胞附着，更高的生存力和增殖，以及相对于HA涂覆的CG底物及其各自未涂覆的（裸露的）形态良好的形态荧光和电子显微镜以及MTT分析所暗示的对应物。免疫荧光研究得出了类似的结论，该研究涉及检查与密集的肌动蛋白应激纤维和纤连蛋白相关的纽蛋白粘着斑接触表达水平，通过SDS-PAGE中的蛋白条带进行蛋白分析以及定量总蛋白测定。细胞反应的增强遵循以下顺序：nHA包被的NG / UFG> nHA包被的CG> NG / UFG> CG底物。预期该研究的结果将解决与具有明显改善的细胞反应的医疗设备具有特定物理和表面特性的纳米结构表面工程相关的挑战。

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