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首页> 外文期刊>Acta biomaterialia >Key role of alkaline phosphatase in the development of human-derived nanoparticles in vitro.
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Key role of alkaline phosphatase in the development of human-derived nanoparticles in vitro.

机译：碱性磷酸酶在体外人类衍生纳米颗粒发育中的关键作用。

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摘要

Alkaline phosphatase (ALP) is an enzyme critical for physiological and pathological biomineralization. Experiments were designed to determine whether ALP participates in the formation of calcifying nanometer sized particles (NPs) in vitro. Filtered homogenates of human calcified carotid artery, aorta and kidney stones were inoculated into cell culture medium containing 10% fetal bovine serum in the absence or presence of inhibitors of ALP or pyrophosphate. A calcific NP biofilm developed within 1 week after inoculation and their development was reduced by pyrophosphate and inhibitors of ALP. ALP protein and enzymatic activity were detected in washed NPs, whether calcified or decalcified. Therefore, ALP activity is required for the formation of calcifying NPs in vitro, as has previously been implicated during pathological calcification in vivo.
机译：碱性磷酸酶（ALP）是对生理和病理生物矿化至关重要的酶。设计实验来确定ALP是否参与体外钙化纳米级颗粒（NPs）的形成。在不存在或存在ALP或焦磷酸盐抑制剂的情况下，将人钙化的颈动脉，主动脉和肾结石的过滤匀浆接种到含有10％胎牛血清的细胞培养基中。接种后1周内形成钙化的NP生物膜，焦磷酸盐和ALP抑制剂可减少钙化NP生物膜的形成。在洗涤的NP中，无论钙化还是脱钙，均检测到ALP蛋白和酶活性。因此，如先前在体内病理性钙化中所暗示的，在体外形成钙化NP需要ALP活性。

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