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首页> 外文期刊>Acta biomaterialia >Antibacterial and cell-adhesive polypeptide and poly(ethylene glycol) hydrogel as a potential scaffold for wound healing
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Antibacterial and cell-adhesive polypeptide and poly(ethylene glycol) hydrogel as a potential scaffold for wound healing

机译：抗菌和细胞粘附性多肽以及聚乙二醇水凝胶可作为伤口愈合的潜在支架

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摘要

The ideal wound-healing scaffold should provide the appropriate physical and mechanical properties to prevent secondary infection, as well as an excellent physiological environment to facilitate cell adhesion, proliferation and/or differentiation. Therefore, we developed a synthetic cell-adhesive polypeptide hydrogel with inherent antibacterial activity. A series of polypeptides, poly(Lys) x(Ala) y (x + y = 100), with varied hydrophobicity via metal-free ring-opening polymerization of NCA-Lys(Boc) and NCA-Ala monomers (NCA = N-carboxylic anhydride) mediated by hexamethyldisilazane (HMDS) were synthesized. These polypeptides were cross-linked with 6-arm polyethylene glycol (PEG)-amide succinimidyl glutarate (ASG) (M w = 10 K) to form hydrogels with a gelation time of five minutes and a storage modulus (G′) of 1400-3000 Pa as characterized by rheometry. The hydrogel formed by cross-linking of poly(Lys) 60(Ala) 40 (5 wt.%) and 6-arm PEG-ASG (16 wt.%) (Gel-III) exhibited cell adhesion and cell proliferation activities superior to other polypeptide hydrogels. In addition, Gel-III displays significant antibacterial activity against Escherichia coli JM109 and Staphylococcus aureus ATCC25923. Thus, we have developed a novel, cell-adhesive hydrogel with inherent antibacterial activity as a potential scaffold for cutaneous wound healing.
机译：理想的伤口愈合支架应提供适当的物理和机械性能以防止继发感染，并提供良好的生理环境以促进细胞粘附，增殖和/或分化。因此，我们开发了具有固有抗菌活性的合成细胞粘附多肽水凝胶。一系列多肽，poly（Lys）x（Ala）y（x + y = 100），通过NCA-Lys（Boc）和NCA-Ala单体的无金属开环聚合而具有不同的疏水性（NCA = N-合成了由六甲基二硅氮烷（HMDS）介导的羧酸酐。这些多肽与6臂聚乙二醇（PEG）-酰胺戊二酸琥珀酰亚胺基酯（ASG）（M w = 10 K）交联以形成水凝胶，凝胶时间为5分钟，储能模量（G'）为1400-流变仪表征为3000 Pa。聚（Lys）60（Ala）40（5 wt。％）和6臂PEG-ASG（16 wt。％）（Gel-III）交联形成的水凝胶表现出的细胞粘附和细胞增殖活性优于其他多肽水凝胶。此外，Gel-III对大肠杆菌JM109和金黄色葡萄球菌ATCC25923具有显着的抗菌活性。因此，我们开发了一种具有固有抗菌活性的新型细胞粘附水凝胶，作为皮肤伤口愈合的潜在支架。

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