• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Acta biomaterialia >Controlled release of vascular endothelial growth factor using poly-lactic-co-glycolic acid microspheres: In vitro characterization and application in polycaprolactone fumarate nerve conduits
【24h】

Controlled release of vascular endothelial growth factor using poly-lactic-co-glycolic acid microspheres: In vitro characterization and application in polycaprolactone fumarate nerve conduits

机译：使用聚乳酸-乙醇酸微球控制释放血管内皮生长因子：富马酸聚己内酯神经导管的体外表征及应用

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Vascular endothelial growth factor (VEGF) is a potent angiogenic stimulator. Controlled release of such stimulators may enhance and guide the vascularization process, and when applied in a nerve conduit may play a role in nerve regeneration. We report the fabrication and in vitro characterization of poly-lactic-co-glycolic acid (PLGA) microspheres encapsulating VEGF and the in vivo application of nerve conduits supplemented with VEGF-containing microspheres. PLGA microspheres containing VEGF were prepared by the double emulsion-solvent evaporation technique. This yielded 83.16% of microspheres with a diameter <53 μm. VEGF content measured by ELISA indicated 93.79 ± 10.64% encapsulation efficiency. Release kinetics were characterized by an initial burst release of 67.6 ± 8.25% within the first 24 h, followed by consistent release of approximately 0.34% per day for 4 weeks. Bioactivity of the released VEGF was tested by human umbilical vein endothelial cell (HUVEC) proliferation assay. VEGF released at all time points enhanced HUVEC proliferation, confirming that VEGF retained its bioactivity throughout the 4 week time period. When the microsphere delivery system was placed in a biosynthetic nerve scaffold robust nerve regeneration was observed. This study established a novel system for controlled release of growth factors and enables in vivo studies of nerve conduits conditioned with this system.
机译：血管内皮生长因子（VEGF）是有效的血管生成刺激剂。此类刺激物的受控释放可以增强和引导血管形成过程，并且当应用于神经导管时，可以在神经再生中发挥作用。我们报道了封装VEGF的聚乳酸-乙醇酸共聚物（PLGA）微球的制备和体外表征，并补充了含VEGF微球的神经导管的体内应用。通过双重乳液-溶剂蒸发技术制备了含有VEGF的PLGA微球。这产生了83.16％的直径＜53μm的微球。通过ELISA测量的VEGF含量表明93.79±10.64％的包封效率。释放动力学的特征是在最初的24小时内初始爆发释放为67.6±8.25％，随后连续4周每天稳定释放约0.34％。通过人脐静脉内皮细胞（HUVEC）增殖测定法测试释放的VEGF的生物活性。在所有时间点释放的VEGF增强HUVEC增殖，证实VEGF在整个4周的时间段内都保留了其生物活性。当将微球递送系统放置在生物合成神经支架中时，观察到健壮的神经再生。这项研究建立了一种控制生长因子释放的新系统，并能够对以该系统为条件的神经导管进行体内研究。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号