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首页> 外文期刊>Acta biomaterialia >Impact of 3-D printed PLA- and chitosan-based scaffolds on human monocyte/macrophage responses: unraveling the effect of 3-D structures on inflammation.
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Impact of 3-D printed PLA- and chitosan-based scaffolds on human monocyte/macrophage responses: unraveling the effect of 3-D structures on inflammation.

机译：3-D打印的基于PLA和壳聚糖的支架对人单核细胞/巨噬细胞反应的影响：阐明3-D结构对炎症的影响。

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Recent studies have pointed towards a decisive role of inflammation in triggering tissue repair and regeneration, while at the same time it is accepted that an exacerbated inflammatory response may lead to rejection of an implant. Within this context, understanding and having the capacity to regulate the inflammatory response elicited by 3-D scaffolds aimed for tissue regeneration is crucial. This work reports on the analysis of the cytokine profile of human monocytes/macrophages in contact with biodegradable 3-D scaffolds with different surface properties, architecture and controlled pore geometry, fabricated by 3-D printing technology. Fabrication processes were optimized to create four different 3-D platforms based on polylactic acid (PLA), PLA/calcium phosphate glass or chitosan. Cytokine secretion and cell morphology of human peripheral blood monocytes allowed to differentiate on the different matrices were analyzed. While all scaffolds supported monocyte/macrophage adhesion and stimulated cytokine production, striking differences between PLA-based and chitosan scaffolds were found, with chitosan eliciting increased secretion of tumor necrosis factor (TNF)-α, while PLA-based scaffolds induced higher production of interleukin (IL)-6, IL-12/23 and IL-10. Even though the material itself induced the biggest differences, the scaffold geometry also impacted on TNF-α and IL-12/23 production, with chitosan scaffolds having larger pores and wider angles leading to a higher secretion of these pro-inflammatory cytokines. These findings strengthen the appropriateness of these 3-D platforms to study modulation of macrophage responses by specific parameters (chemistry, topography, scaffold architecture).
机译：最近的研究指出炎症在触发组织修复和再生中起决定性作用，与此同时，公认的炎症反应加剧可能导致植入物排斥。在此背景下，理解并具有调节针对组织再生的3-D支架引起的炎症反应的能力至关重要。这项工作报告了对人类单核细胞/巨噬细胞与通过3D打印技术制造的具有不同表面特性，结构和受控孔几何形状的可生物降解3D支架接触的细胞因子谱的分析。优化了制造工艺，以创建基于聚乳酸（PLA），PLA /磷酸钙玻璃或壳聚糖的四个不同的3-D平台。分析了允许在不同基质上分化的人外周血单核细胞的细胞因子分泌和细胞形态。尽管所有支架均支持单核细胞/巨噬细胞粘附并刺激细胞因子生成，但发现PLA支架和壳聚糖支架之间存在显着差异，壳聚糖引起肿瘤坏死因子（TNF）-α分泌增加，而PLA支架则诱导白细胞介素生成更高。 （IL）-6，IL-12 / 23和IL-10。即使材料本身引起了最大的差异，支架的几何形状也影响了TNF-α和IL-12 / 23的产生，壳聚糖支架具有较大的孔和更宽的角度，导致这些促炎细胞因子的分泌更高。这些发现增强了这些3-D平台通过特定参数（化学，地形，支架结构）研究巨噬细胞应答调节的适当性。

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