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首页> 外文期刊>RSC Advances >In-depth study on the gene silencing capability of silica nanoparticles with different pore sizes: degree and duration of RNA interference
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In-depth study on the gene silencing capability of silica nanoparticles with different pore sizes: degree and duration of RNA interference

机译：对不同孔径尺寸的二氧化硅纳米粒子基因沉默能力的深入研究：RNA干扰的程度和持续时间

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摘要

The mesoporous silica nanoparticle (MSN) has been utilized for various drug delivery systems. For the efficient loading and functional delivery of bioactive molecules, the pore size should be finely controlled. Here, we synthesize MSNs having different pore sizes and investigate the relationship between the pore size of the MSN and the efficacy and kinetics of gene silencing induced by siRNA loaded MSNs. We found that MSNs with various pore sizes present great differences in gene down-regulation of green fluorescence protein (GFP), vascular endothelial growth factor (VEGF) and hepatitis C virus nonstructural protein 3 (HCV NS3) as well as the endocytosis pathways of MSNs in cancer cells. In specific, MSNs with a 7 and 10 nm pore size show the most significant gene knockdown with a long-lasting effect and great protective effect of the cargo from nuclease-mediated degradation compared to MSNs with a 2, 4 and 23 nm pore size. In addition, although similar amounts of siRNA was delivered inside cells, the degree of gene knockdown was significantly different depending on pore size of the employed MSN. The present study indicates that the porosity in nanoparticles acts as a crucial factor in a potent biomolecule cargo delivery system and the optimized MSN based system should provide better opportunities in the application of porous nanoparticles in biomedical research and clinical applications in the future.
机译：中孔二氧化硅纳米粒子（MSN）已用于各种药物递送系统。为了有效的加载和功能递送生物活性分子，应精细控制孔径。这里，我们合成具有不同孔径尺寸的MSN，并研究MSN的孔径与SiRNA诱导的基因沉默的疗效和动力学之间的关系。我们发现，具有各种孔径的MSNS具有对绿色荧光蛋白（GFP），血管内皮生长因子（VEGF）和丙型肝炎病毒非结构蛋白3（HCV NS3）以及MSN的内吞作用的巨大差异在癌细胞中。在具体的，具有7至10nm孔径的MSN示出具有长效作用和从核酸酶介导的降解相比具有2,4和23纳米孔径的MSN的货物的大保护作用的最显著基因敲除。另外，虽然在细胞内递送了类似的siRNA，但根据所使用的MSN的孔径，基因敲低度显着差异。本研究表明，纳米颗粒中的孔隙率作为有效的生物分子货物输送系统中的关键因素，并且优化的MSN基系统应在未来应用多孔纳米粒子在生物医学研究和临床应用中的应用中提供更好的机会。

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  • 来源
    《RSC Advances》 |2016年第32期|共8页
  • 作者

    Kim Seongchan; Na Hee-Kyung; Won Cheolhee; Min Dal-Hee;

  • 作者单位

    Seoul Natl Univ Dept Chem IBS Ctr RNA Res Seoul 151747 South Korea;

    Seoul Natl Univ Dept Chem IBS Ctr RNA Res Seoul 151747 South Korea;

    Lemonex Inc Inst Nanobio Convergence Technol Seoul 151742 South Korea;

    Seoul Natl Univ Dept Chem IBS Ctr RNA Res Seoul 151747 South Korea;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学;
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