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Control of pH by acetic acid and its effect on ethanol fermentation in an integrated ethanol-methane fermentation process

机译：用乙酸控制pH及其对乙醇 - 甲烷发酵过程中乙醇发酵的影响

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摘要

The integrated ethanol-methane fermentation process employing cassava or other feedstock requires pH control to maximize yields. Sulfuric acid is currently used to adjust the pH of the recycled process water but this causes problems with sulfate-reducing bacteria. To resolve this, acetic acid was evaluated to regulate pH in the range 4.0-5.5. Significant advantages were found and acetic acid toxicity was avoided. The ethanol yield from glucose increased 4.6% on average while production of the undesired byproduct glycerol decreased 52.6%. The ethanol yield per DCW (dry cell weight) increased from 14.0 g g(-1) (sulfuric acid control) to 32.0 g g(-1) when 250 mM acetic acid was present at pH 5.0. At pH 5.5, ethanol yield per DCW increased from 10.5 g g (1) to 23.0 g g (1). In both cases, the ethanol yield more than doubled. Using acetic acid instead of sulfuric acid to control pH thus increased ethanol production while simultaneously reducing undesirable side effects. The results presented here could also provide a useful approach for the management of wastewater in other submerged fermentation industries.
机译：使用木薯或其他原料的集成乙醇 - 甲烷发酵工艺需要pH控制以最大限度地提高产率。硫酸目前用于调节再循环过程水的pH，但这会导致硫酸盐还原细菌的问题。为了解决这一点，评价乙酸以调节4.0-5.5范围内的pH。发现了显着的优点，避免了乙酸毒性。葡萄糖的乙醇产量平均增加4.6％，同时产生不需要的副产品甘油的生产减少了52.6％。当在pH5.0处存在250mM乙酸时，每DCW（干细胞重量）的乙醇产量从14.0g（-1）（硫酸对照）增加到32.0gg（-1）。在pH 5.5时，每DCW的乙醇产率从10.5g g（1）增加到23.0g g（1）。在这两种情况下，乙醇产量增加了一倍多。使用乙酸代替硫酸来控制pH从而增加乙醇生产，同时减少不希望的副作用。此处提出的结果还可以为其他浸没式发酵行业提供废水的有用方法。

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  • 来源
    《RSC Advances》 |2016年第63期|共8页
  • 作者

    Yang Xinchao; Wang Ke; Wang Huijun; Zhang Jianhua; Tang Lei; Mao Zhonggui;

  • 作者单位

    Jiangnan Univ Sch Biotechnol Key Lab Ind Biotechnol Minist Educ Wuxi 214122 Peoples R China;

    Jiangnan Univ Sch Biotechnol Key Lab Ind Biotechnol Minist Educ Wuxi 214122 Peoples R China;

    Jiangnan Univ Sch Biotechnol Key Lab Ind Biotechnol Minist Educ Wuxi 214122 Peoples R China;

    Jiangnan Univ Sch Biotechnol Key Lab Ind Biotechnol Minist Educ Wuxi 214122 Peoples R China;

    Jiangnan Univ Sch Biotechnol Key Lab Ind Biotechnol Minist Educ Wuxi 214122 Peoples R China;

    Jiangnan Univ Sch Biotechnol Key Lab Ind Biotechnol Minist Educ Wuxi 214122 Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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