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首页> 外文期刊>RSC Advances >Carrier-free co-immobilization of xylanase, cellulase and beta-1,3-glucanase as combined cross-linked enzyme aggregates (combi-CLEAs) for one-pot saccharification of sugarcane bagasse
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Carrier-free co-immobilization of xylanase, cellulase and beta-1,3-glucanase as combined cross-linked enzyme aggregates (combi-CLEAs) for one-pot saccharification of sugarcane bagasse

机译：无携带的木聚糖酶，纤维素酶和β-1,3-葡聚糖酶作为组合交联酶聚集体（COMBI-CLEA）的单糖糖糖糖糖糖糖

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摘要

Combined cross-linked enzyme aggregates (combi-CLEAs) are an innovative prospect and a lucrative technology. The present study addresses the preparation, characterization and application of combi-CLEAs with xylanase, cellulase and beta-1,3-glucanase to achieve one-pot bioconversion of lignocellulosic biomass to fermentable sugars. A three-phase partitioning (TPP) method was used to aggregate the enzymes. Glutaraldehyde (100 mM) was employed as a cross-linker with the cross-linking time of 7.5 h. Scanning electron microscopy of the tri-enzyme biocatalyst has a coarse-grained appearance. Combi-CLEAs were more thermally stable, retaining about 70% of their initial activity at 70 degrees C compared to 30% for the free enzyme. The storage stability of combi-CLEAs was more than 97% of their activity after incubation for 11 weeks at 4 degrees C, whereas the free enzymes retained about 65% of initial activity. The residual activity of combi-CLEAs remained constant at 90% until the sixth cycle. Contrary to free enzymes that remain in the hydrolysate, which prevents their recovery, reuse of combi-CLEAs was possible. Free enzymes hydrolyze the ammonia cooked sugarcane bagasse at about 73%, whereas the combi-CLEAs resulted in maximum hydrolysis of about 83.5% in 48 h.
机译：组合的交联酶聚集体（COMBI-CLEA）是一种创新的前景和利润丰厚的技术。本研究解决了与木聚糖酶，纤维素酶和β-1,3-葡聚糖酶组合，表征和应用的组合，表征和应用，以实现木质纤维素生物质的一锅生物转化至可发酵的糖。使用三相分区（TPP）方法来聚集酶。戊二醛（100mm）用作交联剂，交联时间为7.5小时。三酶生物催化剂的扫描电子显微镜具有粗粒外观。 Combi-CleA更热稳定，在70℃下保留约70％的初始活性，而游离酶的30％则为30％。在4摄氏度孵育11周后，Combi-Cla面积的储存稳定性超过其活性的97％，而游离酶保留约65％的初始活性。 Combi-CleA的残余活性在90％之前保持恒定，直到第六周期为90％。与保留在水解产物中的游离酶相反，这防止了它们的恢复，可以重复使用组合物。自由酶在约73％的含量下水解氨煮熟的甘蔗囊肿，而组合物将导致最大水解约83.5％，48小时。

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  • 来源
    《RSC Advances》 |2016年第39期|共9页
  • 作者

    Periyasamy Karthik; Santhalembi Laishram; Mortha Gerard; Aurousseau Marc; Subramanian Sivanesan;

  • 作者单位

    Anna Univ Environm Management Lab Dept Appl Sci &

    Technol Madras 600025 Tamil Nadu India;

    Anna Univ Dept Biotechnol Madras 600025 Tamil Nadu India;

    Univ Grenoble Alpes LGP2 F-38000 Grenoble France;

    Univ Grenoble Alpes LGP2 F-38000 Grenoble France;

    Anna Univ Environm Management Lab Dept Appl Sci &

    Technol Madras 600025 Tamil Nadu India;

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  • 正文语种 eng
  • 中图分类 化学;
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