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A biocompatible cerasome based platform for direct electrochemistry of cholesterol oxidase and cholesterol sensing

机译：一种基于生物相容性的胆固醇氧化酶直接电化学和胆固醇感应平台

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摘要

Cerasomes are a novel organic-inorganic hybrid material composed of spherical lipid bilayer vesicles with an internal aqueous compartment, which are similar to liposomes formed from phospholipids, with an inorganic silicate framework covering the vesicular surface. In this research, an anionic cerasome formed from N,N-dihexadecyl-N'-[(3-triethoxysilyl)propyl]urea was successfully prepared, and the cerasomes were characterized by scanning electron microscopy (SEM), behaving as a spherical-like structure. An electrochemical platform was constructed using a combination of the cerasomes and cholesterol oxidase (ChOx) on a glassy carbon electrode. Ultraviolet-visible (UV-vis) spectroscopy was used to monitor the assembly process and the electrochemical impedance spectroscopy (EIS) results demonstrated that ChOx had been successfully immobilized. The obtained enzyme-modified electrode exhibited both the effective direct electron transfer between the enzyme and electrode surface and the excellent electrochemical catalytic activity towards cholesterol with a wide linear range from 5.0 x 10(-6) to 3.0 x 10(-3) mol L-1 and a low detection limit 1.7 x 10(-6) mol L-1 (S/N 3). The excellent catalytic performance of the modified electrode is attributed to the good biocompatibility of the cerasomes, which can provide a soft and morphologically stable interface for enzymatic immobilization, allowing the enzyme to retain its catalytic activity, along with their specific affinity (Km 0.139 mmol L-1) for water-insoluble cholesterol. The results indicate that cerasomes are useful as a platform for electrochemical sensing of cholesterol and have the potential to immobilize enzymes for bioelectrochemical applications.
机译：Cerasomes是一种新的有机无机杂化材料，其由具有内部水隔室的球形脂质双层囊泡组成，其与由磷脂形成的脂质体类似，覆盖囊状表面的无机硅酸盐框架。在该研究中，成功​​制备了由N，N-二异癸基-N'-[（3-三乙氧基甲硅烷基）丙基]尿素形成的阴离子Carasome，并通过扫描电子显微镜（SEM）来表征该方法，表现为球形样品结构体。使用Cerasomes和胆固醇氧化酶（CHOX）的组合构建电化学平台在玻璃状碳电极上构建。使用紫外 - 可见（UV-VI）光谱法监测组装过程，电化学阻抗光谱（EIS）结果表明CHOX已成功固定。所得酶改性电极表现出酶和电极表面之间的有效直接电子转移，以及较宽的线性范围为5.0×10（ - 6）至3.0×10（-3）摩尔L的胆固醇之间的优异电化学催化活性。 -1和低检测限1.7×10（-6）mol L-1（S / N 3）。改性电极的优异催化性能归因于治疗方法的良好生物相容性，其可以提供酶活化的柔软和形态稳定的界面，使酶与其特定亲和力一起保留其催化活性（KM 0.139mmol L. -1）用于水不溶性胆固醇。结果表明，治愈物可用作胆固醇的电化学传感平台，并且具有固定生物电化学应用的酶的潜力。

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  • 来源
    《RSC Advances》 |2016年第75期|共10页
  • 作者

    Wu Shuyao; Chen Jiali; Liu Daliang; Zhuang Qian; Pei Qi; Xia Lixin; Zhang Qian; Kikuchi Jun-ichi; Hisaeda Yoshio; Song Xi-Ming;

  • 作者单位

    Liaoning Univ Coll Chem Liaoning Key Lab Green Synth &

    Preparat Chem Adv Shenyang 110036 Peoples R China;

    Liaoning Univ Coll Chem Liaoning Key Lab Green Synth &

    Preparat Chem Adv Shenyang 110036 Peoples R China;

    Liaoning Univ Coll Chem Liaoning Key Lab Green Synth &

    Preparat Chem Adv Shenyang 110036 Peoples R China;

    Liaoning Univ Coll Chem Liaoning Key Lab Green Synth &

    Preparat Chem Adv Shenyang 110036 Peoples R China;

    Liaoning Univ Coll Chem Liaoning Key Lab Green Synth &

    Preparat Chem Adv Shenyang 110036 Peoples R China;

    Liaoning Univ Coll Chem Shenyang 110036 Peoples R China;

    Liaoning Univ Coll Chem Shenyang 110036 Peoples R China;

    Nara Inst Sci &

    Technol Grad Sch Mat Sci 8916-5 Takayama Ikoma Nara 6300192 Japan;

    Kyushu Univ Grad Sch Engn Dept Chem &

    Biochem Nishi Ku 744 Motooka Fukuoka 8190395 Japan;

    Liaoning Univ Coll Chem Liaoning Key Lab Green Synth &

    Preparat Chem Adv Shenyang 110036 Peoples R China;

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