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首页> 外文期刊>RSC Advances >New insights into the qualitative phenolic profile of Ficus carica L. fruits and leaves from Tunisia using ultra-high-performance liquid chromatography coupled to quadrupole-time-of-flight mass spectrometry and their antioxidant activity
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New insights into the qualitative phenolic profile of Ficus carica L. fruits and leaves from Tunisia using ultra-high-performance liquid chromatography coupled to quadrupole-time-of-flight mass spectrometry and their antioxidant activity

机译：使用超高效液相色谱法与四轴 - 飞行时间质谱和抗氧化活性使用超高效液相色谱法与突尼斯的曲线植物和叶片定性酚醛概念的新见解。及其抗氧化活性

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Ficus carica L. fruits have been consumed from the earliest times, and other parts of the tree have been used for traditional medicinal purposes. Nowadays, the beneficial properties of this and other Ficus species are attributed to the presence of key phytochemicals. To increase our knowledge about this topic, the present study has conducted phenolic profiling of the leaves and whole fruits from two Tunisian cultivars, 'Temri' and 'Tounsi', using reversed-phase ultra-high-performance liquid chromatography (RP-UHPLC) coupled to two detection systems: diode-array detection (DAD) and quadrupole time-of-flight (QTOF) mass spectrometry (MS). UV-Vis absorption was a valuable tool for classifying phenolic compounds into families, while MS using electrospray ionization (ESI) and MS/MS allowed the molecular formula to be established and structural information to be obtained. The total phenol content and the antioxidant activity were also assessed. As a result, in the negative ionization mode 91 phenolic compounds were characterized including hydroxybenzoic acids, hydroxycinnamic acids, hydroxycoumarins and flavanoids (flavonols, flavones, flavanones, flavanonols, flavanols and isoflavones). This work was complemented by the detection of other 18 phenolic compounds in the positive ionization mode, including anthocyanins and furanocoumarins. To the best of our knowledge, this is the first time most of these compounds have been tentatively reported in Ficus carica. These results indicate the complexity of this family of secondary metabolites in Ficus carica, as well as the potential of this analytical method for characterization purposes. In conclusion, the qualitative phenolic profile, total phenolic content and antioxidant activity differed especially between leaves and fruits.
机译：Ficus Carica L.果实从最早的次消费，树的其他部分已被用于传统药用目的。如今，这种榕属的有益特性归因于关键植物化学的存在。为了提高关于这一主题的知识，本研究采用了两种突尼斯品种，“Temri”和'Tounsi'对叶片和整个水果进行了酚醛剖析，使用反相超高效液相色谱（RP-UHPLC）耦合到两个检测系统：二极管阵列检测（爸爸）和四极杆飞行时间（QTOF）质谱（MS）。 UV-Vis吸收是将酚类化合物分类为家族的有价值的工具，而MS使用电喷雾电离（ESI）和MS / MS允许建立分子式并获得结构信息。还评估了总酚含量和抗氧化活性。结果，在负电离模式中，91种酚类化合物的特征在于羟基苯甲酸，羟基氨基酸，羟基呋喃和黄烷酮（黄酮醇，黄酮，黄烷酮，黄兰酮，黄烷醇和异黄酮）。通过在阳性电离模式中检测其他18个酚类化合物，包括花青素和Furanocoumarins，通过检测到这种作品。据我们所知，这是大多数这些化合物的第一次在Ficus Carica中报告。这些结果表明，这种分析方法的次级代谢物在杂草中的次级代谢物的复杂性。总之，定性酚醛概况，总酚类含量和抗氧化活性尤其不同于叶片和水果之间。

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