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首页> 外文期刊>RSC Advances >Graphene oxide-based Fe3O4 nanoparticles as a novel scaffold for the immobilization of porcine pancreatic lipase
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Graphene oxide-based Fe3O4 nanoparticles as a novel scaffold for the immobilization of porcine pancreatic lipase

机译：基于石墨烯氧化物的Fe3O4纳米颗粒作为一种用于固定猪胰脂肪酶的新型支架

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摘要

Nano- and hybrid materials have recently emerged as a new approach for improving enzyme activity and stability, and are suitable for commercial applications. In this paper, graphene oxide-based magnetic hybrids were prepared successfully. A chloropropyl-functionalized graphene oxide decorated with Fe3O4 nanoparticles was made, denoted as CPS/GO-Fe3O4@ MCM-41. This was characterized by X-ray powder diffraction, scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, vibrating sample magnetometry, thermogravimetry and N-2 adsorption/desorption. Then, porcine pancreas lipase (PPL) was immobilized onto the graphene oxide-based magnetic nanoparticles via covalent bonding. The results show that the novel supporting material CPS/GO-Fe3O4@ MCM-41 was the best for PPL immobilization compared to two other nanomaterials (GO-Fe3O4@ MCM-41 and GO-Fe3O4). The supporting material CPS/GO-Fe3O4@ MCM-41 exhibited enhanced immobilization efficiency (up to 98%), maximum relative activity (up to 97.9%), high stability and reusability (85% after 56 d and 87% after 10 cycles respectively, both at 30 degrees C). Additionally, it offered some other advantages, such as easy recycling and reuse, complying with the requirements of green chemistry. Therefore, it is proposed that CPS/GO-Fe3O4@ MCM-41 provides a new approach for commercial applications.
机译：纳米和杂化材料最近被出现为改善酶活性和稳定性的新方法，适用于商业应用。在本文中，成功制备了石墨烯氧化物基磁性杂交物。制备用Fe3O4纳米颗粒装饰的氯丙基官能化的氧化物，表示为CPS / GO-FE3O4 @ MCM-41。这由X射线粉末衍射，扫描电子显微镜，透射电子显微镜，傅里叶变换红外光谱，振动样品磁体，热重试验和N-2吸附/解吸。然后，通过共价键合将猪胰腺脂肪酶（PPL）固定在石墨烯氧化物类磁性纳米颗粒上。结果表明，与另外两个纳米材料相比，新型支撑材料CPS / GO-FE3O4 @ MCM-41是PPL固定的最佳方法（GO-FE3O4 @ MCM-41和GO-FE3O4）。支撑材料CPS / GO-FE3O4 @ MCM-41表现出增强的固定效率（高达98％），最大相对活性（高达97.9％），高稳定性和可重用性（分别在56天后85％和87％） ，均为30℃）。此外，它还提供了一些其他优点，如容易回收和重用，符合绿色化学的要求。因此，建议CPS / GO-FE3O4 @ MCM-41为商业应用提供了一种新的方法。

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  • 来源
    《RSC Advances》 |2015年第126期|共13页
  • 作者

    Shao Yongbo; Jing Tao; Tian Jingzhi; Zheng Yongjie;

  • 作者单位

    Qiqihar Univ Coll Chem &

    Chem Engn Qiqihar 161006 Peoples R China;

    Qiqihar Univ Coll Chem &

    Chem Engn Qiqihar 161006 Peoples R China;

    Qiqihar Univ Coll Chem &

    Chem Engn Qiqihar 161006 Peoples R China;

    Qiqihar Univ Coll Chem &

    Chem Engn Qiqihar 161006 Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学;
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