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首页> 外文期刊>Acta biomaterialia >Cyclo-DfKRG peptide modulates in vitro and in vivo behavior of human osteoprogenitor cells on titanium alloys.
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Cyclo-DfKRG peptide modulates in vitro and in vivo behavior of human osteoprogenitor cells on titanium alloys.

机译：Cyclo-DfKRG肽在钛合金上调节人骨祖细胞的体外和体内行为。

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摘要

The first aim of the present study was to investigate the capacity of a cyclo-DfKRG-coated hydroxyapatite-titanium alloy (Ti-HA-RGD) to activate in vitro human osteoprogenitor cells adhesion and differentiation. The second purpose was to examine in vivo the role of a autologous cell seeding on cyclo-DfKRG-functionalized materials to provide bone repair after implantation in femoral condyle of rabbits. Our in vitro results have demonstrated that both titanium alloy functionalized with hydroxyapatite (Ti-HA-RGD and Ti-HA) contributed to higher cell adhesion than titanium alloy alone respectively 85 and 55% vs 15% compared to tissue culture polystyrene after one hour of cell seeding. As for differentiation, after 3 days of culture, Ti-HA presented the highest increase of ALP mRNA of all surfaces studied. Ti-HA-RGD showed an intermediate value about half as high as Ti-HA. Moreover after 3 days, both Ti-HA and Ti-HA-RGD surfaces showed the highest increase of cbfa1 mRNA expression. Two weeks following implantation, in vivo findings revealed that percentage of lacunae contact observed with pre-cellularized Ti-HA-RGD samples remains significantly lower than with Ti-HA group (10.5+/-9.6 % vs 33.7+/-11.5 %, P<0.03). Meanwhile, RGD peptide coating had no significant additional effect on the bone implant contact and area. Moreover, histomorphometry analysis revealed that implantation of pre-cellularized RGD coated materials with ROP cells increased significantly peri-implant fibrous area (24+/-11.6% vs 3+/-1.7% for Ti-HA-RGD, P<0.02). RGD coatings demonstrated osteoblastic adhesion, differentiation and in vivo bone regeneration at most equivalent to HA coatings.
机译：本研究的第一个目的是研究环DfKR​​G涂层的羟基磷灰石钛合金（Ti-HA-RGD）激活体外人骨祖细胞粘附和分化的能力。第二个目的是在体内检查自体细胞接种在环DfKR​​G功能化材料上以在兔股骨con植入后提供骨修复的作用。我们的体外研究结果表明，用羟基磷灰石功能化的两种钛合金（Ti-HA-RGD和Ti-HA）与单独使用钛合金相比，与组织培养的聚苯乙烯相比，分别比单独的钛合金分别具有85和55％和15％的较高细胞粘附力。细胞接种。至于分化，在培养3天后，Ti-HA表现出所有研究表面中ALP mRNA的最高增加。 Ti-HA-RGD的中间值约为Ti-HA的一半。此外，三天后，Ti-HA和Ti-HA-RGD表面均显示出cbfa1 mRNA表达的最高增加。植入后两周，体内发现表明，预细胞化的Ti-HA-RGD样品观察到的腔隙接触百分比仍显着低于Ti-HA组（10.5 +/- 9.6％vs 33.7 +/- 11.5％，P <0.03）。同时，RGD肽涂层对骨植入物的接触和面积没有明显的额外影响。此外，组织形态计量学分析显示，用ROP细胞植入预先细胞化的RGD涂层材料可显着增加植入物周围的纤维面积（24-11.6％，而Ti-HA-RGD为3 +/- 1.7％，P <0.02）。 RGD涂层表现出的成骨细胞粘附力，分化能力和体内骨骼再生能力与HA涂层相当。

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