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Low cost semi-continuous bioprocess and online monitoring of hydrogen production from crude glycerol

机译：低成本半连续生物过程和粗甘油从氢生产的在线监测

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摘要

High process costs arising from expensive media components, hydrogen partial pressure buildup in the head-space of the reactor, sharp decrease in medium pH due to accumulation of organic acids, substrate inhibition, as well as loss of biomass due to short hydraulic retention times are the major bottlenecks of fermentative hydrogen production. Therefore, the purpose of the present investigation was to develop a process to deal with all these problems at a time. The proposed approach demonstrates enhanced hydrogen production by a low cost process involving crude glycerol (CG), the waste from the biodiesel production process, as the only feedstock. This semi-continuous type of fermentation carried out in a 7.5 L bioreactor helped to eliminate substrate inhibition with no compromise in cumulative hydrogen production. By controlling product inhibition and the negative effect of by-product accumulation, as well as by preventing the loss of active biomass, a production of 5.18 L-H2 per L-medium has been achieved. This amount is higher than the 2.02 to 2.68 L-H2 per Lmedium previously reported for CG bioconversion. Reduction of feed CG concentration from 120 g L~1 to 60 g L~1 was found to improve the glycerol utilization from 65% to 91%.
机译：从昂贵的介质组分产生高的过程成本，反应器的头部空间中的氢气部分压力累积，由于有机酸的积聚，底物抑制以及由于液压保留时间的储存，介质pH的急剧降低发酵氢生产的主要瓶颈。因此，本调查的目的是制定一次处理所有这些问题的过程。所提出的方法通过涉及粗甘油（CG）的低成本过程，表明了增强的氢气产生，从生物柴油生产过程中的废物，作为唯一的原料。在7.5L生物反应器中进行的这种半连续发酵有助于消除累积氢气产生的底物抑制。通过控制产物抑制和副产物积累的负面影响，以及通过防止活性生物质的损失，已经实现了每L-培养基的5.18L-H2。此金额高于先前报告的CG生物转化的每Lmedium 2.02至2.68 L-H2。发现进料CG浓度从120g 1〜1至60g L〜1的浓度从65％的65％提高甘油利用率。

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  • 来源
    《RSC Advances》 |2015年第109期|共8页
  • 作者

    Saurabh Jyoti Sarma; Sampa Maiti; Satinder Kaur Brar;

  • 作者单位

    Institut National de la Recherche Scientique (INRS) Centre Eau Terre &

    Environnement 490 Rue de la Couronne Quebec(QC) G1K 9A9 Canada.;

    Institut National de la Recherche Scientique (INRS) Centre Eau Terre &

    Environnement 490 Rue de la Couronne Quebec(QC) G1K 9A9 Canada.;

    Institut National de la Recherche Scientique (INRS) Centre Eau Terre &

    Environnement 490 Rue de la Couronne Quebec(QC) G1K 9A9 Canada.;

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  • 正文语种 eng
  • 中图分类 化学;
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