• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>RSC Advances >Apigenin mediated gold nanoparticle synthesis and their anti-cancer effect on human epidermoid carcinoma (A431) cells
【24h】

Apigenin mediated gold nanoparticle synthesis and their anti-cancer effect on human epidermoid carcinoma (A431) cells

机译：Apigenin介导的金纳米粒子合成及其对人表皮癌（A431）细胞的抗癌作用

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We report that the flavonoid, apigenin (4',5,7,-trihydroxyflavone) is able to form apigenin linked gold nanoparticles (ap-AuNPs) at RT with apigenin itself acting as the stabilizing agent. The synthesized ap-AuNPs have been characterised by UV-Visible spectroscopy, HR-TEM, DLS, FTIR and TGA analyses. The biocompatible nature of ap-AuNPs is shown by their non-toxicity towards normal epidermoid cells (HaCat). Additionally, they are shown to exhibit anti-cancer activity towards epidermoid squamous carcinoma cells (A431). The uptake of ap-AuNPs into A431 cells is seen by TEM. Apoptosis induced by ap-AuNPs in these cells is observed through EtBr/AO and DAPI staining. Flow cytometric results also reveal the occurrence of early apoptotic cells, late apoptotic cells and the presence of sub-G1 population. DNA prepared from ap-AuNPs treated A-431 cells reveals a ladder like pattern in agarose gel electrophoresis indicating oligonucleosomal cleavage which is a hallmark of apoptosis. The ap-AuNPs also inhibit angiogenesis as shown by Chick chorioallantoic membrane (CAM) assay. Taking the results together, we believe that ap-AuNPs show promise in treatment of skin cancer. The ap-AuNPs also have cytotoxic potential against the human cervical squamous cell carcinoma cell line SiHa.
机译：我们报告称，黄酮类化合物，Apigenin（4'，5,7，-Thydroxyflavone）能够在室温下形成具有Apigenin本身作为稳定剂的Apigenin连接的金纳米粒子（AP-AUNP）。合成的AP-AUNPS的特征在于UV可见光谱，HR-TEM，DLS，FTIR和TGA分析。 AP-AUNP的生物相容性本质由它们对正常表皮细胞（HACAT）的非毒性表示。另外，它们被证明表现出对表皮鳞状癌细胞（A431）的抗癌活性。通过TEM看到AP-AUNP进入A431细胞的摄取。通过ETBR / AO和DAPI染色观察到这些细胞中的AP-AUNP诱导的细胞凋亡。流式细胞术结果还揭示了早期凋亡细胞，晚期凋亡细胞和亚g1种群的存在的发生。由AP-AUNPS处理的A-431细胞制备的DNA揭示了琼脂糖凝胶电泳中的梯形图，表明寡核科裂解是凋亡的标志。 AP-AUNPS还抑制血管生成，如鸡绒铃膜（CAM）测定所示。将结果携手共同，我们认为AP-AUNPS显示治疗皮肤癌症的承诺。 AP-AUNPS还针对人宫颈鳞状细胞癌细胞系Siha具有细胞毒性潜力。

著录项

  • 来源
    《RSC Advances》 |2015年第63期|共12页
  • 作者

    Rajendran Indra; Dhandapani Harini; Anantanarayanan Rajaram; Rajaram Rama;

  • 作者单位

    Cent Leather Res Inst Dept Biochem Madras 600020 Tamil Nadu India;

    Cent Leather Res Inst Dept Biochem Madras 600020 Tamil Nadu India;

    Cent Leather Res Inst Dept Biophys Chennai 600020 Tamil Nadu India;

    Cent Leather Res Inst Dept Biochem Madras 600020 Tamil Nadu India;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学;
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号