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首页> 外文期刊>Acta crystallographica, Section F. Structural biology and crystallization communications >Expression, purification, crystallization and preliminary X-ray analysis of tannase from Lactobacillus plantarum
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Expression, purification, crystallization and preliminary X-ray analysis of tannase from Lactobacillus plantarum

机译：植物乳杆菌鞣酸酶的表达，纯化，结晶和初步X射线分析

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摘要

Tannase catalyses the hydrolysis of the galloyl ester bond of tannins to release gallic acid. It belongs to the serine esterases and has wide applications in the food, feed, beverage, pharmaceutical and chemical industries. The tannase from Lactobacillus plantarum was cloned, expressed and purified. The protein was crystallized by the sitting-drop vapour-diffusion method with microseeding. The crystals belonged to space group P1, with unit-cell paramters a = 46.5, b = 62.8, c = 83.8 angstrom, alpha = 70.4, beta = 86.0, gamma = 79.4 degrees. Although the enzyme exists mainly as a monomer in solution, it forms a dimer in the asymmetric unit of the crystal. The crystals diffracted to beyond 1.60 angstrom resolution using synchrotron radiation and a complete data set was collected to 1.65 angstrom resolution.
机译：鞣酸催化单宁的没食子酸酯键的水解以释放没食子酸。它属于丝氨酸酯酶，在食品，饲料，饮料，制药和化学工业中具有广泛的应用。克隆，表达和纯化了来自植物乳杆菌的鞣酸酶。通过坐滴蒸汽扩散法和微播种使蛋白质结晶。晶体属于空间群P1，其晶胞参数a = 46.5，b = 62.8，c = 83.8埃，α= 70.4，β= 86.0，γ= 79.4度。尽管酶主要以单体形式存在于溶液中，但它在晶体的不对称单元中形成二聚体。使用同步加速器辐射将晶体衍射至超过1.60埃的分辨率，并收集了至1.65埃分辨率的完整数据集。

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