Cloning, expression, purification, crystallization and preliminary crystallographic analysis of the kinase domain of AtMAP4Kalpha2 from Arabidopsis thaliana

Shen Liqiang; Du Xiaoli; Su Qing; Li Mingxia; Zhou Zhiqin

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Cloning, expression, purification, crystallization and preliminary crystallographic analysis of the kinase domain of AtMAP4Kalpha2 from Arabidopsis thaliana

机译：拟南芥AtMAP4Kalpha2激酶结构域的克隆，表达，纯化，结晶和初步晶体学分析

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Arabidopsis thaliana (At) MAP4Kalpha2, a member of the Ste20/PAK-like protein kinase family, is an essential component of the septum initiation network involved in cell division. To better understand the mode of action of AtMAP4Kalpha2, a structural biology approach has been pursued. In this study, the kinase domain of AtMAP4Kalpha2 was cloned, expressed, purified and crystallized. The crystals diffracted to 1.9 angstrom resolution and belonged to space group C222(1), with unit-cell parameters a = 55.27, b = 82.93, c = 133.15 angstrom.

机译：拟南芥（At）MAP4Kalpha2，是Ste20 / PAK样蛋白激酶家族的成员，是参与细胞分裂的隔膜起始网络的重要组成部分。为了更好地理解AtMAP4Kalpha2的作用方式，已寻求一种结构生物学方法。在这项研究中，AtMAP4Kalpha2的激酶域被克隆，表达，纯化和结晶。晶体衍射到1.9埃分辨率，属于C222（1）空间群，单位晶胞参数a = 55.27，b = 82.93，c = 133.15埃。

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《Acta crystallographica, Section F. Structural biology and crystallization communications》 |2013年第7期|共4页
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Shen Liqiang; Du Xiaoli; Su Qing; Li Mingxia; Zhou Zhiqin;
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中图分类晶体学;
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1. Cloning, expression, purification, crystallization and preliminary crystallographic analysis of the kinase domain of AtMAP4Kalpha2 from Arabidopsis thaliana [J] . Shen Liqiang, Du Xiaoli, Su Qing, Acta crystallographica, Section F. Structural biology and crystallization communications . 2013,第7期

机译：拟南芥AtMAP4Kalpha2激酶结构域的克隆，表达，纯化，结晶和初步晶体学分析
2. GPX3 from Arabidopsis thaliana: cloning, expression, purification, crystallization and preliminary X-ray analysis [J] . Li Kun, Yang Qingzhan, Wang Wei, Acta crystallographica, Section F. Structural biology and crystallization communications . 2013,第11期

机译：拟南芥GPX3：克隆，表达，纯化，结晶和初步X射线分析
3. Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of AHP2, a signal transmitter protein from Arabidopsis thaliana [J] . Degtjarik Oksana, Dopitova Radka, Puehringer Sandra, Acta crystallographica, Section F. Structural biology and crystallization communications . 2013,第2期

机译：拟南芥信号传递蛋白AHP2的克隆，表达，纯化，结晶和初步X射线衍射分析
4. Clone in vitro expression and functional analysis of the cDNA encoding a putative nucleoside transporter from arabidopsis thaliana [C] . Li Jian, Wang Daowen International conference on engineering and technological sciences;ICETS2000 . 2000

机译：拟南芥核苷转运蛋白编码cDNA的克隆体外表达及功能分析
5. Expression, purification, crystallization and preliminary X-ray analysis of PR-AMP cyclohydrolase, a zinc binding enzyme from Methanobacterium thermoautotrophicum. [D] . Boju, Lorena. 2004

机译：PR-AMP环水解酶的表达，纯化，结晶和初步X射线分析，PR-AMP环水解酶是一种来自嗜热自养甲烷杆菌的锌结合酶。
6. Cloning expression purification crystallization and preliminary crystallographic analysis of the kinase domain of AtMAP4Kalpha2 from Arabidopsis thaliana [O] . Liqiang Shen, Xiaoli Du, Qing Su, 2013

机译：拟南芥AtMAP4Kalpha2激酶结构域的克隆表达纯化结晶和初步晶体学分析
7. Cloning, expression, purification, crystallization and preliminary crystallographic analysis of the kinase domain of AtMAP4Kalpha2 fromArabidopsis thaliana [O] . Liqiang Shen, Xiaoli Du, Qing Su, 2013

机译：近距离静脉内塔巴氏菌的激酶结构域的克隆，表达，纯化，结晶和初步结晶分析

Cloning, expression, purification, crystallization and preliminary crystallographic analysis of the kinase domain of AtMAP4Kalpha2 from Arabidopsis thaliana

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