Expression, purification and crystallization of the FP domain of the human F-box protein Fbxo7

Shang Jinsai; Wang Guan; Yang Yang; Huang Xiaolan; Du Zhihua

首页> 外文期刊>Acta crystallographica, Section F. Structural biology and crystallization communications >Expression, purification and crystallization of the FP domain of the human F-box protein Fbxo7

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Expression, purification and crystallization of the FP domain of the human F-box protein Fbxo7

机译：人F-box蛋白Fbxo7的FP结构域的表达，纯化和结晶

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Fbxo7 is a conserved protein in higher eukaryotes that belongs to the F-box protein family. Fbxo7 is the substrate-recognition component of the SCFFbxo7 (Skp1-Cul1-Fbox protein) E3 ubiquitin ligase. Besides the F-box motif, Fbxo7 also contains a C-terminal proline-rich region, an N-terminal ubiquitin-like domain and a novel FP (Fbxo7/PI31) domain preceding the F-box motif. The FP domains of Fbxo7 and the PI31 proteasome inhibitor mediate interaction between the two proteins. For structure determination of the FP domain of Fxbo7, a protein construct (amino acids 181-335) corresponding to the FP domain was expressed, purified and crystallized. The native and selenomethionine-labeled proteins crystallized in different crystal forms. Native and single-wavelength anomalous dispersion data sets with diffraction to 2.1 and 2.0 angstrom resolution, respectively, have been collected and structure determination is in progress.

机译：Fbxo7是高级真核生物中的保守蛋白，属于F-box蛋白家族。 Fbxo7是SCFFbxo7（Skp1-Cul1-Fbox蛋白）E3泛素连接酶的底物识别成分。除F-box基序外，Fbxo7还包含一个C端富含脯氨酸的区域，一个N端泛素样结构域和一个位于F-box基序之前的新型FP（Fbxo7 / PI31）域。 Fbxo7的FP域和PI31蛋白酶体抑制剂介导这两种蛋白之间的相互作用。为了确定Fxbo7的FP结构域，表达，纯化和结晶对应于FP结构域的蛋白质构建体（氨基酸181-335）。天然和硒代蛋氨酸标记的蛋白质以不同的晶体形式结晶。已经收集了分别衍射到2.1和2.0埃分辨率的原始和单波长异常色散数据集，并且正在进行结构确定。

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《Acta crystallographica, Section F. Structural biology and crystallization communications》 |2013年第10期|共3页
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Shang Jinsai; Wang Guan; Yang Yang; Huang Xiaolan; Du Zhihua;
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1. Expression, purification and crystallization of the FP domain of the human F-box protein Fbxo7 [J] . Shang Jinsai, Wang Guan, Yang Yang, Acta crystallographica, Section F. Structural biology and crystallization communications . 2013,第10期

机译：人F-box蛋白Fbxo7的FP结构域的表达，纯化和结晶
2. The FP domains of PI31 and Fbxo7 have the same protein fold but very different modes of protein-protein interaction [J] . Shang Jinsai, Huang Xiaolan, Du Zhihua Journal of Biomolecular Structure and Dynamics . 2015,第7a8期

机译：PI31和FBXO7的FP结构域具有相同的蛋白质折叠，但蛋白质 - 蛋白质相互作用的非常不同的模式
3. The F-box protein Fbxo7 interacts with human inhibitor of apoptosis protein cIAP1 and promotes cIAP1 ubiquitination [J] . Chang YF, Cheng CM, Chang LK, Biochemical and Biophysical Research Communications . 2006,第4期

机译：F-box蛋白Fbxo7与人类凋亡抑制蛋白cIAP1相互作用并促进cIAP1泛素化
4. Expression of Recombinant Human Bone Morphogenetic Protein 2 in Insect Cells, Purification and Activity Analysis [C] . Wen Chu, Shenheng Luo, Lihong Xu, International conference on applied biotechnology . 2014

机译：重组人骨形态发生蛋白2在昆虫细胞中的表达，纯化及活性分析
5. Expression, purification and crystallization of the C-terminal domain of human ubiquitin specific protease 7. [D] . Zheng, Hong. 2010

机译：人泛素特异性蛋白酶7 C末端结构域的表达，纯化和结晶。
6. Expression purification and crystallization of the FP domain of the human F-box protein Fbxo7 [O] . Jinsai Shang, Guan Wang, Yang Yang, 2013

机译：人F-box蛋白Fbxo7的FP结构域的表达纯化和结晶
7. Structure of a conserved dimerization domain within the F-box protein Fbxo7 and the PI31 proteasome inhibitor [O] . Kirk, R., Laman, H., Knowles, P.P., 2008

机译：F-box蛋白Fbxo7和PI31蛋白酶体抑制剂内保守的二聚结构域的结构

Expression, purification and crystallization of the FP domain of the human F-box protein Fbxo7

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