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首页> 外文期刊>Acta crystallographica, Section F. Structural biology and crystallization communications >Crystallization and preliminary X-ray analysis of human leukocyte cell-derived chemotaxin 2 (LECT2)
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Crystallization and preliminary X-ray analysis of human leukocyte cell-derived chemotaxin 2 (LECT2)

机译：人白细胞衍生的趋化因子2（LECT2）的结晶和初步X射线分析

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摘要

Human leukocyte cell-derived chemotaxin 2 (LECT2) is a chemotactic factor for neutrophils that plays multifunctional roles in liver regeneration, regulation of neuritic development and proliferation of chondrocytes and osteoblasts. In addition, the C-terminal region of LECT2 belongs to the zinc metalloendopeptidase M23 (PF01551) family. Purified LECT2 was crystallized using the sitting-drop vapour-diffusion method at 293K. Crystals of selenomethionine-substituted LECT2 that diffracted X-rays to 1.94 angstrom resolution were obtained using a reservoir solution consisting of 0.2M ammonium sulfate, 0.1M HEPES pH 7.5, 25%(w/v) PEG 8000. The crystal belonged to space group P212121, with unit-cell parameters a = 59.4, b = 63.5, c = 64.0 angstrom. The calculated Matthews coefficient (VM = 2.10 angstrom 3Da1, solvent content 40%) indicates that the crystal consists of two molecules per asymmetric unit.
机译：人白细胞衍生的趋化因子2（LECT2）是嗜中性粒细胞的趋化因子，在肝再生，调节神经元发育以及软骨细胞和成骨细胞的增殖中发挥多功能作用。此外，LECT2的C端区域属于锌金属内肽酶M23（PF01551）家族。使用坐滴蒸气扩散法在293K结晶纯化的LECT2。使用由0.2M硫酸铵，0.1M HEPES pH 7.5、25％（w / v）PEG 8000组成的储库溶液，获得X射线衍射至1.94埃分辨率的硒甲硫氨酸取代的LECT2晶体。该晶体属于空间群P212121，其晶胞参数a = 59.4，b = 63.5，c = 64.0埃。计算出的马修斯系数（VM = 2.10埃3Da1，溶剂含量40％）表明该晶体由每个不对称单元两个分子组成。

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