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首页> 外文期刊>Gene: An International Journal Focusing on Gene Cloning and Gene Structure and Function >Identification and expression analysis of the leukocyte cell-derived chemotaxin-2 (LECT2) gene in duck (Anas platyrhynchos)
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Identification and expression analysis of the leukocyte cell-derived chemotaxin-2 (LECT2) gene in duck (Anas platyrhynchos)

机译：鸭子白细胞细胞衍生的趋化蛋白-2（Lect2）基因的鉴定及表达分析（Anas platyrhynchos）

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摘要

Leukocyte cell-derived chemotaxin 2 (LECT2), first identified as a chemotactic factor, is involved in the regulation of liver regeneration, carcinogenesis, and natural killer T-cell homeostasis in mammals. The function of LECT2 in the duck remains unclear, however. A suppression subtractive cDNA library was constructed from the livers of 3-day-old ducklings treated with duck hepatitis virus type I (DHV-1). A total of 66 expressed sequence tags (ESTs) were identified in the libraries. Among the novel gene fragments identified was the LECT2 gene. Full-length duck LECT2 (duLECT2) complementary DNA (cDNA) was obtained using the reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of the cDNA ends (RACE). The cDNA consisted of a 50 nucleotide 5' untranslated region (UTR), an 84 nucleotide 3' UTR, and a 1020 nucleotide open reading frame encoding a single protein of 339 amino acids. In agreement with a previously reported LECT2 sequence, the predicted amino add sequence contains characteristic phosphorylation and N-glycosylation sites. DuLECT2 is highly similar to LECT2 genes from other vertebrates. Phylogenetic analysis demonstrated that the LECT2 gene has been highly conserved throughout vertebrate evolution. RT-PCR analyses revealed that duLECT2 mRNA is widely expressed in healthy tissues. They also showed that duLECT2 mRNA is significantly up-regulated in the liver and spleen following injection with DHV-1 or polyriboinosinic polyribocytidylic acid (poly I:C), peaking 4 or 12 h post-challenge in the liver and spleen, respectively, and afterwards gradually returning to normal. Our findings suggest that duLECT2 contributes to the innate immune response against viral infections. (C) 2013 Elsevier B.V. All rights reserved.
机译：白细胞细胞衍生的趋化酶2（Lect2）首先被鉴定为趋化因子，参与哺乳动物中肝再生，致癌物和天然杀伤T细胞稳态的调节。然而，Lect2在鸭子中的功能仍然尚不清楚。抑制减去CDNA文库由用鸭肝炎病毒I型（DHV-1）处理的3天古老的鸭鱼。在文库中鉴定了总共66个表达序列标签（EST）。在鉴定的新型基因片段中，是Lect2基因。使用逆转录聚合酶链反应（RT-PCR）获得全长鸭Lect2（Dulect2）互补DNA（cDNA），并快速扩增cDNA末端（种族）。 CDNA由50个核苷酸5'未转过来的区域（UTR），84个核苷酸3'UTR，以及编码339个氨基酸的单一蛋白质的1020个核苷酸开放阅读框。在与先前报道的Lect2序列的同意中，预测的氨基加法序列含有特征磷酸化和N-糖基化位点。 Dulect2与来自其他脊椎动物的Lect2基因高度相似。系统发育分析证明，牙科术基因在整个脊椎动物进化中得到了高度保守。 RT-PCR分析显示Dult2 mRNA在健康组织中广泛表达。他们还表明，在用DHV-1或多吡吡吡吡吡吡啶鎓多嗪酸（Poly I：C）中，分别在肝脏和脾脏的攻击后达到4或12小时后，Dult2 mRNA在肝脏和脾脏中显着上调。之后逐渐恢复正常。我们的研究结果表明Dulect2有助于针对病毒感染的先天免疫应答。 （c）2013年elestvier b.v.保留所有权利。

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  • 来源
  • 作者

    Xu Qi; Chen Yang; Tong Yi Yu; Huang Zheng Yang; Zhao Wen Ming; Duan Xiu Jun; Zhang Yang; Li Xiu; Chang Guo Bin; Chen Guo Hong;

  • 作者单位

    Yangzhou Univ Key Lab Anim Genet &

    Breeding &

    Mol Design Jiangs Yangzhou Peoples R China;

    Yangzhou Univ Key Lab Anim Genet &

    Breeding &

    Mol Design Jiangs Yangzhou Peoples R China;

    Yangzhou Univ Key Lab Anim Genet &

    Breeding &

    Mol Design Jiangs Yangzhou Peoples R China;

    Yangzhou Univ Key Lab Anim Genet &

    Breeding &

    Mol Design Jiangs Yangzhou Peoples R China;

    Yangzhou Univ Key Lab Anim Genet &

    Breeding &

    Mol Design Jiangs Yangzhou Peoples R China;

    Chinese Waterfowl Germplasrn Resource Pool Taizhou Peoples R China;

    Yangzhou Univ Key Lab Anim Genet &

    Breeding &

    Mol Design Jiangs Yangzhou Peoples R China;

    Yangzhou Univ Key Lab Anim Genet &

    Breeding &

    Mol Design Jiangs Yangzhou Peoples R China;

    Yangzhou Univ Key Lab Anim Genet &

    Breeding &

    Mol Design Jiangs Yangzhou Peoples R China;

    Yangzhou Univ Key Lab Anim Genet &

    Breeding &

    Mol Design Jiangs Yangzhou Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 医学遗传学;
  • 关键词

    Duck; duLECT2; Gene expression; DHV-1;

    机译：鸭;Dulect2;基因表达;DHV-1;

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