Crystallization of the C-terminal domain of the bacteriophage T5 L-shaped fibre

Garcia-Doval Carmela; Luque Daniel; Caston Jose R.; Boulanger Pascale; van Raaij Mark J.

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Crystallization of the C-terminal domain of the bacteriophage T5 L-shaped fibre

机译：噬菌体T5 L形纤维C端结构域的结晶

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Tails of bacteriophage T5 (a member of the Siphoviridae family) were studied by electron microscopy. For the distal parts of the L-shaped tail fibres, which are involved in host cell receptor binding, a low-resolution volume was calculated. Several C-terminal fragments of the fibre were expressed and purified. Crystals of two of them were obtained that belonged to space groups P6(3) and R32 and diffracted synchrotron radiation to 2.3 and 2.9 angstrom resolution, respectively. A single-wavelength anomalous dispersion data set to 2.5 angstrom resolution was also collected from a selenomethionine-derivatized crystal of one of the fragments, which belonged to space group C2.

机译：通过电子显微镜研究了噬菌体T5的尾巴（Siphoviridae家族的成员）。对于参与宿主细胞受体结合的L形尾纤维的远端部分，计算了低分辨率体积。表达并纯化了纤维的几个C端片段。获得了其中两个的晶体，它们分别属于空间群P6（3）和R32，并分别将同步加速器辐射衍射到2.3和2.9埃分辨率。还从属于空间组C2的一个片段的硒代蛋氨酸衍生的晶体中收集了设置为2.5埃分辨率的单波长异常色散数据。

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《Acta crystallographica, Section F. Structural biology and crystallization communications》 |2013年第12期|共5页
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Garcia-Doval Carmela; Luque Daniel; Caston Jose R.; Boulanger Pascale; van Raaij Mark J.;
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正文语种 eng
中图分类晶体学;
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1. Crystallization of the C-terminal domain of the bacteriophage T5 L-shaped fibre [J] . Garcia-Doval Carmela, Luque Daniel, Caston Jose R., Acta crystallographica, Section F. Structural biology and crystallization communications . 2013,第12期

机译：噬菌体T5 L形纤维C端结构域的结晶
2. Crystallization of the C-terminal head domain of the fibre protein from a siadenovirus, turkey adenovirus 3 [J] . Singh Abhimanyu K., Ballmann Monika Z., Benko Maria, Acta crystallographica, Section F. Structural biology and crystallization communications . 2013,第10期

机译：唾液腺病毒，火鸡腺病毒3的纤维蛋白C末端头部结构域的结晶
3. Crystallization of the C-terminal domain of the fibre protein from snake adenovirus 1, an atadenovirus [J] . Singh Abhimanyu K., Menendez-Conejero Rosa, San Martin Carmen, Acta crystallographica, Section F. Structural biology and crystallization communications . 2013,第12期

机译：蛇腺病毒1（一种腺病毒）中纤维蛋白C末端结构域的结晶
4. The N-and C-terminal carbohydrate recognition domains of galectin-9 contribute differently to its multiple functions in innate immunity and adaptive immunity [C] . Yurong Li, Hui Peng, Gencheng Han, International conference of molecular simulations and applied informatics technologies . 2012

机译：galectin-9的N和C端碳水化合物识别结构域对其先天免疫和适应性免疫的多种功能有不同的贡献
5. Expression, purification and crystallization of the C-terminal domain of human ubiquitin specific protease 7. [D] . Zheng, Hong. 2010

机译：人泛素特异性蛋白酶7 C末端结构域的表达，纯化和结晶。
6. Crystallization of the C-terminal domain of the bacteriophage T5 L-shaped fibre [O] . Carmela Garcia-Doval, Daniel Luque, José R. Castón, 2013

机译：噬菌体T5 L形纤维C端结构域的结晶
7. Structure of the receptor-binding carboxy-terminal domain of the bacteriophage T5 L-shaped tail fibre with and without its intra-molecular chaperone [O] . Garcia-Doval, Carmela, Castón, José R., Luque, Daniel, 2016

机译：带有和不带有分子内伴侣的噬菌体T5 L形尾纤维的受体结合羧基末端结构域
8. Replication of Linear Duplex DNA in Vitro with Bacteriophage T5 DNA Polymerase [R] . Fujimura, R. K., Das, S. K., Allison, D. P., 1980

机译：用噬菌体T5 DNa聚合酶体外复制线性双链DNa

Crystallization of the C-terminal domain of the bacteriophage T5 L-shaped fibre

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