Crystallization and preliminary X- ray crystallographic analysis of the curli transporter CsgG

Goyal Parveen; Van Gerven Nani; Jonckheere Wim; Remaut Han

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Crystallization and preliminary X- ray crystallographic analysis of the curli transporter CsgG

机译：卷曲运输蛋白CsgG的结晶和初步X射线晶体学分析

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Gram-negative bacteria have eight known protein secretion systems. The type-VIII secretion system, also known as the curli biosynthesis system, is responsible for the formation of aggregative fibres known in Escherichia coli as curli. Curli are extracellular proteinaceous fibres primarily involved in bacterial biofilm formation and attachment to nonbiotic surfaces. The secretion of curli subunits depends on a dedicated lipoprotein, CsgG, which is found to form an oligomeric secretion channel in the outer membrane. A nonlipidated mutant of CsgG was expressed and crystallized in a soluble form. The crystals diffracted to 3.15 angstrom resolution and belong to space group P1 with a unit cell containing a predicted 16 molecules per asymmetric unit.

机译：革兰氏阴性细菌具有八个已知的蛋白质分泌系统。 VIII型分泌系统，也称为curli生物合成系统，负责形成大肠杆菌中称为curli的聚集纤维。 Curli是细胞外蛋白质纤维，主要参与细菌生物膜的形成以及与非生物表面的附着。 curli亚基的分泌取决于专用的脂蛋白CsgG，发现其在外膜中形成寡聚分泌通道。 CsgG的非脂质突变体被表达并以可溶形式结晶。晶体衍射至3.15埃分辨率，属于空间群P1，其晶胞每个不对称单元包含16个预测分子。

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《Acta crystallographica, Section F. Structural biology and crystallization communications》 |2013年第12期|共5页
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Goyal Parveen; Van Gerven Nani; Jonckheere Wim; Remaut Han;
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1. Crystallization and preliminary X- ray crystallographic analysis of the curli transporter CsgG [J] . Goyal Parveen, Van Gerven Nani, Jonckheere Wim, Acta crystallographica, Section F. Structural biology and crystallization communications . 2013,第12期

机译：卷曲运输蛋白CsgG的结晶和初步X射线晶体学分析
2. Complex assembly, crystallization and preliminary X-ray crystallographic analysis of duck MHC class I complexed with a TUMV viral peptide [J] . Liu Zixin, Xie Xiaoli, Li Zhuolin, Research in Veterinary Science . 2020,第1期

机译：鸭MHC类I复合的复杂组装，结晶和初步X射线晶体分析，其与TumV病毒肽复合
3. Purification, crystallization and preliminary X-ray crystallographic analysis of the phosphatase domain (PA3346PD) of the response regulator PA3346 from Pseudomonas aeruginosa PAO1 [J] . Chen Li-Ying, Wu Pei-Hsun, Guan Hong-Hsiang, Acta crystallographica. Section F, Structural biology communications . 2015,第4期

机译：铜绿假单胞菌PAO1反应调节剂PA3346磷酸酶结构域（PA3346PD）的纯化，结晶和初步X射线晶体学分析
4. Preliminary X-ray crystallographic analysis of MntC, a periplasmic manganese binding component of a Mn transporter from Synechocystis sp. PCC 6803 [C] . Anati R., Bhattacharyya- Pakrasi M., Pakrasi H. B., International Congress on Photosynthesis . 2001

机译：MNTC的初步X射线晶体分析，SneCechocystis Sp的Mn转运蛋白的周质锰结合组分。 PCC 6803.
5. Expression, purification, crystallization and preliminary X-ray analysis of PR-AMP cyclohydrolase, a zinc binding enzyme from Methanobacterium thermoautotrophicum. [D] . Boju, Lorena. 2004

机译：PR-AMP环水解酶的表达，纯化，结晶和初步X射线分析，PR-AMP环水解酶是一种来自嗜热自养甲烷杆菌的锌结合酶。
6. Crystallization and preliminary X-ray crystallographic analysis of the curli transporter CsgG [O] . Parveen Goyal, Nani Van Gerven, Wim Jonckheere, 2013

机译：卷曲转运蛋白CsgG的结晶和初步X射线晶体学分析
7. Crystallization and preliminary X-ray crystallographic analysis of the curli transporter CsgG [O] . Parveen Goyal, Nani Van Gerven, Wim Jonckheere, 2013

机译：CURLI转运蛋白CSGG结晶和初步X射线晶体分析

Crystallization and preliminary X- ray crystallographic analysis of the curli transporter CsgG

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