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首页> 外文期刊>Biochemical Genetics >Analysis of Genetic Diversity and Development of a SCAR Marker for Green Tea (Camellia sinensis) Cultivars in Zhejiang Province: The Most Famous Green Tea-Producing Area in China
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Analysis of Genetic Diversity and Development of a SCAR Marker for Green Tea (Camellia sinensis) Cultivars in Zhejiang Province: The Most Famous Green Tea-Producing Area in China

机译：浙江省绿茶（Camellia Sinensis）品种遗传多样性及遗传多样性及发展：中国最着名的绿茶区

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摘要

Camellia sinensis (L.) O. Kuntze is one of the most important non-alcoholic beverage crops in Asian and African countries. In recent years, many green tea cultivars have been released and played an important role in improving the production and quality of tea trees. The objectives of this study were to assess the genetic diversity of the eighteen main green tea cultivars in Zhejiang Province-the most famous green tea-producing area of China-using start codon-targeted (SCoT) markers and to develop a specific sequence-characterized amplified region (SCAR) marker for application in cultivar diagnosis. Thirty-one SCoT primers produced 264 loci, 226 of which were polymorphic. The genetic similarity coefficients among these green tea cultivars ranged from 0.587 to 0.814, indicating that a high level of genetic diversity was present. Both a UPGMA dendrogram and a PCoA plot grouped the tea cultivars into three groups. The partitioning of groups in the UPGMA and PCoA was similar, and much of the clustering was highly consistent with the classification of tea cultivars according to their genetic backgrounds. A unique SCoT band, SCoT4-1649, specific to the tea cultivar Yingshuang,' was transformed into a SCAR marker. This SCAR marker is highly useful for the identification and germplasm conservation of green tea cultivars.
机译：Camellia Sinensis（L.）O. Kuntze是亚洲和非洲国家最重要的非酒精饮料作物之一。近年来，许多绿茶品种已被释放，并在提高茶树的生产和质量方面发挥了重要作用。本研究的目标是评估浙江省十八份主要绿茶品种的遗传多样性 - 中国最着名的绿茶生产区 - 使用起始密码子有针对性（Scot）标记，并开发特定的序列特征扩增区域（瘢痕）标志物，用于在品种诊断中的应用。三十一突出的引物产生264个基因座，其中226个是多态的。这些绿茶品种之间的遗传相似系数范围为0.587至0.814，表明存在高水平的遗传多样性。 UPGMA树枝图和PCOA图均分为三组。在UPGMA和PCOA中的基团分区是相似的，并且许多聚类与根据其遗传背景的茶草品种的分类非常一致。一个独特的苏格兰乐队，Scot4-1649，特定于茶叶yingshuang，'被转化为疤痕标记。这种瘢痕标记物对绿茶品种的鉴定和种质保护非常有用。

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  • 来源
    《Biochemical Genetics》 |2019年第4期|共16页
  • 作者

    Xu Yan-Xia; Shen Si-Yan; Chen Wei; Chen Liang;

  • 作者单位

    Chinese Acad Agr Sci Tea Res Inst Natl Ctr Tea Improvement Minist Agr Key Lab Tea Biol &

    Chinese Acad Agr Sci Tea Res Inst Natl Ctr Tea Improvement Minist Agr Key Lab Tea Biol &

    Chinese Acad Agr Sci Tea Res Inst Natl Ctr Tea Improvement Minist Agr Key Lab Tea Biol &

    Chinese Acad Agr Sci Tea Res Inst Natl Ctr Tea Improvement Minist Agr Key Lab Tea Biol &

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 遗传学;
  • 关键词

    Tea plant; Varieties; SCoT marker; Genetic diversity; SCAR marker;

    机译：茶厂;品种;苏格兰标记;遗传多样性;疤痕标记;

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